槐花中总黄酮的提取及单体分离提纯研究

【作者】 兰昌云；

【导师】 范必威；

【作者基本信息】 成都理工大学 ， 分析化学， 2006， 硕士

【摘要】 本文主要对槐花中总黄酮的提取以及总黄酮单体—芦丁和槲皮素的分离提纯进行了研究。 本文研究了超声波辅助提取法，得出了超声波法辅助提取槐花中总黄酮的最佳工艺条件。在最佳工艺条件下，超声波法连续两次提取，其黄酮相对提取率可达99％以上(索氏提取10h至无色所得槐花中总黄酮量为8.456％，作为槐花的总黄酮量)；与常规热回流法相比，提取率明显提高，而且时间缩短了4-8倍。同时还研究了采用大孔吸附树脂对总黄酮提取液进行纯化处理的技术。结果表明D4020型非极性大孔吸附树脂对槐花中的总黄酮有较好的吸附和解吸效果，最大吸附量为6.82mg／mL，解吸率达90％以上，解吸后所得到的总黄酮粗品色泽好、芦丁含量较高。 本文还研究了将总黄酮粗品进一步纯化直接得到芦丁样品和对总黄酮粗品进行水解制备槲皮素的工艺条件，最后了得到纯度较高的芦丁和槲皮素样品。芦丁的纯度可达96％以上、槲皮素单体纯度可达97.5％以上，达到了药典规定样品可直接用作药和作为化学试剂的标准。 本文对槐花进行实验的研究，具有黄酮提取率高、提取时间短、所得产品纯度高等优点，为综合开发该资源提供了有益的参考。更多还原

【Abstract】 We mainly research on the flavone extraction of the Sophora japonica and the separation of rutin and quercetin in this study.Through researching on the assistant ultrasonic extraction, We find the best technics in extracting flavone with ultrasonic. In the best technics, the relatively rate of flavone is more 99% after extracting twice with ultrasonic. The rate of flavone is 8.456% in extracting 10 hours with Soxhlet extraction, and it is rregarded as the total flavonoids content of Sophora japonica. The extraction rate of flavone with ultrasonic evidently increase, and the extraction time shortened four to eight times. We also research that the macroporous adsorptive resin purified the flavone extracted solution. The results of experiment indicated, the D4020 macroporous adsorptive resin has prefertable adsorption and desorption effect on the flavone of Sophora japonica, and the most asorption reached 6.82mg/mL, the rate of desorption reached more 90%. The colour and lustre of the flavone crude product was good, the content of rutin was high.Researching on the technics conditions that the flavone crude products were made of the rutin product through more purifying and the quecetin product through hydrolyzing, We finally gained the high purity rutin and quercetin products. The purity of rutin was more 96%, and the purity of quercetin was more 97.5%. They reached the standard of making chemistry reagent and directly making medicine according to the codex.We research on the experiments of Sophora japonica in this text. They had many benefits, for example high extraction rate of flavone, short extraction time, high purity of product, and so on. This provide profitable referrence for empoldering Sophora japonica resources.更多还原