【作者】 孙毅；

【导师】 沈异凡； 毛培宏；

【作者基本信息】 新疆大学 ， 原子与分子物理， 2006， 硕士

【摘要】 纤维素是地球上数量最大的可再生性能源物质,微生物对它的降解、转化是自然界碳素循环的重要环节,对这个过程的有效利用可望在能源,饲料和食物的持续供应上发挥巨大的作用。正是基于这种思想,世界各国微生物工作者前赴后继,对产生纤维素酶的微生物进行了广泛深入的研究。本研究首次应用低能氮离子注入和激光照射进行纤维素酶产生菌绿色木霉的育种研究,通过测定和分析其经诱变后产生的纤维素酶系中三种酶的活性,分析这两种诱变手段对绿色木霉的生物效应,筛选出高产纤维素酶的绿色木霉突变菌株。通过本研究,获得了8株纤维素酶酶活较高的绿色木霉突变菌株,其中突变菌株L181的CMC酶活和突变菌株56的FPA酶活均比出发菌株提高了一倍以上,达到了本试验的预期目标。同时,也注意到了变异菌株除酶活变化外的一些物理性状上的变化。在对试验结果进行分析的同时,也对实验中出现的一些现象和问题进行了讨论。在研究过程中,我们自行设计了简易激光照射装置,并采用了照射菌膜的激光方法,体现了本研究的创新性、科学性和实用性,可供相关研究借鉴。更多还原

【Abstract】 The cellulose is the biggest renewable energy source material on the earth, its degradation and conversion by microorganism are very important for carbon cycle in nature, moreover, the efficient utilization of this process may develop the enormous function on supplying energy、feedstuff and food. Base on this idea, International community microorganism worker work hard for studying microorganism which produce cellulose.This research first applied the N~+ implantation of low energy and laser technology to the research of the breeding of the cellulose enzyme production strain Trichoderma viride. We analyzed the biologic effects that the two methods applying to the Trichoderma viride through measuring and analyzing the activation of three enzymes in the cellulose serial enzymes produced by the mutant strains. Finally, we chose the mutant strains which can produce cellulose greatly.Through the research, we obtained 8 mutant strains. Among them, CMC enzyme activation of mutant strain L181 and FPA enzyme activation of mutant strain 56 all increase above a time compared with the original strain, the anticipated target of the reseach has been achieved. At the same time, the physical changes of the mutant strains has been noticed and discussd also. In the research process, we have independently designed a simple laser irradiation unit, and has used the new method which to irradiate the mycoderma of Trichoderma virid with laser beam directly. It has manifested originality、scientificalness and practicability of the reseach and may supply the correlation research too.更多还原