【作者】 严涛；

【导师】 李冠；

【作者基本信息】 新疆大学 ， 植物学， 2006， 硕士

【摘要】 将离子束技术应用于食用菌的诱变育种研究，以猴头菌为实验材料，在较大的剂量范围对猴头菌丝进行注入，研究离子束的剂量与猴头菌的生长速度，多糖含量和总氨基酸含量的关系，为猴头菌的诱变育种找到一个新的；可行的方法，为离子束进一步应用于其它食用菌的诱变育种提供参考。 在1×10¹⁵N⁺／cm²—8×10¹⁶N⁺／cm²剂量范围内对猴头菌丝进行注入，统计分析注入后猴头菌丝生长速度的变化，发现猴头菌丝的生长速度随N离子剂量的增加呈先上升，后下降，再上升，再下降的“M型曲线”变化；发现脉冲时间对离子注入的结果有影响，脉冲时间从小到大的变化对猴头菌丝生长速度的影响呈先下降，后上升，再下降的“马鞍型曲线”。 将注入后生长速度较快的菌株继续传代培养，得到了3株最适温度下生长速度较快的变异菌株和3株较高温度下生长速度较快的变异菌株，检测这6株变异株的多糖含量，总氨基酸含量，发现变异株的多糖含量和总氨基酸含量与对照比较均有不同程度的提高，其中，多糖含量最高提高了224.42％，并且菌丝的多糖含量与菌丝的生长速度呈正相关；总氨基酸含量最高提高了104.04％。 变异菌株的菇实验，也验证了变异株生长速度快的结论。更多还原

【Abstract】 This mutagenic study of Hericium erinaceus by ion beam tries to prove that it is different from other mutagenic agent when ion beam is used for domestic fungus mutation breeding and provide a reference foundation data for using the ion beam in other domestic fungus. Based on the outcome of the study, it is hoped to confirm a new, advisable, physical mutagen for domestic fungus mutation breeding.The growth speed of the Hericium erinaceus hypha is observed and analyzed after N⁺ ion implantation within the range from 1×10¹⁵N⁺/cm² to 8×10¹⁶N⁺/cm². The result shows the growth speed of Hericium erinaceus hypha presents an "M shape" curve, namely, the growth speed rises, then descends, again rises and again descends. It is also found that the pulse time obviously influences the effect of N⁺ ion implantation. When the time increases, the growth speed of Hericium erinaceus hypha presents a "saddle shape" curve, which means that the growth speed descends first, rises then and descends again.Six variation strains whose growth speeds are higher than that of control are obtained through this study. Measuring their polysaccharide contents and their total amino acid contents, it is found that the polysaccharide content and the total amino acid content are also higher than that of control, and that the relation between the polysaccharide contents and the growth speeds are of positive correlation更多还原