【作者】 张曹民；

【导师】 李劲松；

【作者基本信息】 南京农业大学 ， 预防兽医学， 2005， 硕士

【摘要】 为了解上海市规模化猪场的猪流感(SI)的流行状况，我们于2001～2002年对上海市郊区部分猪场进行了SI的流行状况调查、血清学调查和病毒分离鉴定。 对上海市6个区县的76个猪场的SI流行状况进行了调查，证实上海地区的养猪场普遍发生或流行过SI，并往往会继发或并发其它疾病，给养猪业造成一定的经济损失。 对6个区(县)的25个猪场进行了SI血清学调查，采集了500份血清样品，用血凝抑制(HI)试验和酶联免疫吸附试验(ELISA)对血清样品进行了检测，发现猪血清中存在SI H1和H3病毒抗体。HI试验检测SI H1病毒抗体的阳性率为47％，H3病毒抗体阳性率为3.6％；ELISA试验检测SI H1病毒抗体的阳性率为57％。种猪血清抗体的阳性率及抗体效价明显高于肉猪和保育猪。 从6个区(县)12个猪场采集到192头猪的鼻拭子，通过鸡胚接种分离到3株病毒分离物，经鉴定为SI H1病毒。更多还原

【Abstract】 In order to understand the epidemic situation of the swine influenza(SI) of the scale pig farm of Shanghai, we carried on epidemiology of SI and serological investigation to some pig farms of suburb county of Shanghai in 2001-2002, and the virus was isolated and identified.76 pig farms of 6 counties in Shanghai were investigated the epidemic state , the results verified that pig farms of Shanghai were generally taking place or had occurred SI, which often induced other diseases simultaneously to continue and caused certain economic losses to swine industry.Serological investigation was carried on to 25 pig farms of 6 counties of Shanghai , 500 serum samples were gathered and detected by the method of HI and ELISA. The results proofed that SI HI and H3 virus antibody existed in the pig serum. HI results suggested that SI HI virus antibody positive rate was 47%, H3 virus antibody positive rate 3.6%. ELISA results suggested that SI HI virus antibody positive rate was 57%. The positive of serum antibody of boars was obviously higher than pork pigs and child care pigs.The nose swabs of 192 pigs were gathered from 12 pig farms of 6 counties, three virus strains were separated through inoculating chicken embryos, and were determined SI H1 virus.更多还原