【作者】 杨安强；

【导师】 潘晓琳；

【作者基本信息】 石河子大学 ， 病理学与病理生理学， 2006， 硕士

【摘要】 目的:检测新疆维吾尔族宫颈癌中p21Ser31Arg和p53Arg72Pro基因型的分布、HPV16 DNA及P21、P53蛋白表达,研究探讨p21Ser31Arg和p53Arg72Pro多态性与新疆维吾尔族宫颈癌遗传易感性的关系及P21和P53蛋白表达与宫颈癌致癌机制的关系。方法:应用PCR、semi-nested PCR、PCR-RFLP技术对100例维吾尔族宫颈癌和100例维吾尔族正常宫颈组织中p21Ser31Arg基因型的分布,152例维吾尔族宫颈癌和110例维吾尔族正常宫颈组织中p53Arg72Pro基因型的分布及HPV16DNA进行检测;应用免疫组化技术检测79例维吾尔族宫颈癌和40例维吾尔族正常宫颈组织中的P21和P53蛋白表达。结果:(1) 100例维吾尔族宫颈癌组中p21Ser31Arg三种基因型的检出率分别为Arg/Arg 13.0%(13/100)、Ser/Ser 38.0%(38/100)、Arg/Ser 49.0%(49/100),对照组(维吾尔族正常宫颈组织)三种基因型的检出率分别为Arg/Arg10.0%(10/100)、Ser/Ser39.0%(39/100)、Arg/Ser51.0%(51/100),两组基因型构成比经统计学分析差异无显著性(χ~2=0.444,P=0.801)。(2) 152例维吾尔族宫颈癌组中p53Arg72Pro三种基因型的检出率分别为Arg/Arg 46.1%(70/152)、Pro/Pro 13.1%(20/152)、Arg/Pro40.8%(62/152),与对照组Arg/Arg30.9%(34/110)、Pro/Pro14.5%(16/110)、Arg/Pro54.6%(60/110)相比,基因型构成比差异有显著性(χ~2=6.307,P=0.041)。(3)宫颈癌组中HPV16DNA检出率为74.3%(113/152),明显高于对照组3.6%(4/110),差异有显著性(χ~2=126.25,P=0.000)。(4)维族宫颈癌组中P21蛋白阳性表达率96.2%(76/79),明显高于对照组5.0%(14/40),两者在统计学上差异有显著性(χ~2=50.70,P=0.000)。(5)宫颈癌组中P53蛋白阳性率为68.4%(54/79),明显高于对照组12.5%(5/40),两者在统计学上差异有显著性(χ~2=30.94,P=0.000)。结论:(1) p21Ser31Arg多态性可能与维吾尔族宫颈癌的发生无明显相关性。(2)更多还原

【Abstract】 Objective: To determine the distribution of p21Ser31Arg, p53Arg72Pro polymorphism and HPV16 DNA and correlate these two polymorphism to genetic susceptibility in HPV -associated cervical carcinoma in Uigur women in Xinjiang. To detect expression of P21 and P53 and explore the carcinogenesis of cervical carcinoma.Methods: PCR ,semi-nested PCR and PCR-RFLP were performed to detected the genotypes distribution of p21Ser31Arg, p53Arg72Pro and HPV16DNA in 152 cases of cervical carcinoma and 110 cases of normal cervical tissue in Uigur women in Xinjiang. Immunohistochemistry was used to detect the expression of P21 and P53 in 79 cases of cervical carcinoma and 40 cases of normal cervical tissue.Results: (1) frequencies of Arg/Arg, Ser/Ser and Arg/Ser in p21 were 13.0% (13/100), 38.0% (38/100) and 49.0%(49/100) in 100 cases of cervical carcinoma and 10.0%(10/100), 39.0%(39/100) and 51%(51/100) in control cases, respectively. Difference of genotype frequencies between the cervical carcinomas and the controls was not significant statistically (χ~2=0.444 , P=0.801).(2) frequencies of Arg/Arg, Pro/Pro and Arg/Pro in p53 were 46.1%(70/152), 13.1% (20/152) and 40.8%(62/152) in 152 cases of cervical carcinoma cases and 30.9%(34/110), 14.54%(16/110) and 54.55%(60/110) in control cases, respectively. Difference of genotype frequencies between the cervical carcinomas and the controls was significant statistically (χ~2=6.307,P=0.041).(3) In cervical carcinoma, the frequency of HPV16DNA of detection was 74.3%(113/152), which was significantly higher than those in control group 3.6%(4/110).Difference of HPV16 positive rate between the cervical carcinomas and the controls was significant statistically (χ~2=126.25,P=0.000) .(4) The positive rate of P21 protein was 96.2%(76/79) in cervical carcinoma, which was higher significantly than those in control group 35.0%(14/40). Difference of P21 protein positive rate更多还原