【作者】 胡轩；

【导师】 焦奎；

【作者基本信息】 青岛科技大学 ， 物理化学， 2006， 硕士

【摘要】 本论文以电化学和紫外-可见光谱方法研究了两种有机染料小分子孔雀石绿和吡哕红B与dsDNA的相互作用。 论文分为四个部分。第一章综述了电化学方法研究有机染料以及抗癌药物等与DNA作用的研究进展。第二、三章分别使用玻碳电极和滴汞电极研究了孔雀石绿与DNA的相互作用。实验证明孔雀石绿与dsDNA可形成复合物，复合物的电位在玻碳电极上发生负移，呈现静电作用的特征。dsDNA修饰电极的结果显示孔雀石绿与DNA通过静电和嵌插作用两种模式相结合，但主要通过嵌插作用相结合。由于DNA在玻碳电极上没有吸附，结合与DNA外部骨架的孔雀石绿更易于在玻碳电极上反应，所以玻碳电极的结果反映静电模式。孔雀石绿与DNA形成复合物后会导致电流减小，可以此建立dsDNA的电分析方法。线性范围为10.0～100.0mg／L，检出限为6.0mg／L。使用滴汞电极对玻碳电极的结果进行验证，在存在dsDNA时，孔雀石绿在滴汞电极上的还原峰峰电位正移，证明DNA与孔雀石绿以嵌插作用结合。在滴汞电极上，以孔雀石绿为探针的DNA定量分析线性范围0.8～12.0mg／L，检出限为0.46mg／L(3σ)。第四部分研究了吡哕红B电化学性质及与dsDNA的相互作用。在玻碳电极上研究了吡哕红B的电化学行为并推导了吡罗红B的电氧化机理。吡哕红B与DNA通过嵌插作用相结合，结合DNA后吡哕红B峰电流明显增大。研究发现吡哕红B的峰电位与DNA鸟嘌呤的峰电位基本重合，因此推测峰电流增大的原因是由于吡哕红B的氧化引起与吡罗红B接触的鸟嘌呤的氧化。更多还原

【Abstract】 The electrochemical and spectrometric studies on the interactions of two organic dye molecules, malachite green (MG) and pyronine B (PB), with DNA have been described in this paper.There are four parts in this paper. In the first part, the development of electrochemical studies of organic molecules interactions with DNA, such as organic dyes and anticancer drugs, have been reviewed. In the second and the third parts, the interactions of malachite green with DNA have been investigated by carbon glassy electrode (GCE) and drop mercury electrode (DME), respectively. The malachite green can bind DNA and form DNA-MG complex. The formal potential of DNA-MG complex shifts negatively at the glassy carbon electrode, which is the character of an electrostatic interaction. The results of dsDNA-modified electrode suggest that the malachite green can bind DNA by two modes and the predominant interaction is intercalation interaction. Because the DNA has no significant adsorption at the GCE, the electro-reaction of malachite green binding with the outer negatively charged DNA phosphate occurs more easily at the surface of GCE than the intercalation mode, so the results of experiments at the GCE appear an electrostatic interaction of dye with DNA. The decrease of the peak current of MG after adding dsDNA was further used for the quantification of dsDNA. The linear range for dsDNA is in the range of 10.0~100.0 mg/L and the detection limit of 6.0 mg/L (3σ). Further studies of MG binding with DNA have been done by drop mercury electrode. After the addition of dsDNA into MG solution, the reductive peak current decreased with a positive shift of the peak potential, which was the typical characteristic of intercalation. The decrease of the peak current was linear with the concentration of dsDNA over the range of 0.8~12.0 mg/L and the detection limit of 0.46 mg/L (3σ).The fourth part focuses on the electrochemical properties of pyronine B (PB) and its更多还原