【作者】 栾美丽；

【导师】 袁其朋；

【作者基本信息】 北京化工大学 ， 生物化工， 2006， 硕士

【摘要】 辅酶Q₁₀(Coenzyme Q₁₀，简写为CoQ₁₀)，又称泛醌，在自然界中分布广泛，主要存在于酵母、植物叶子和种子及动物的心脏、肝脏和肾脏中。辅酶Q₁₀是细胞呼吸链上的一种递氢体，是细胞自身产生的天然抗氧化剂、细胞代谢激活剂。具有抗氧化性、消除自由基、提高机体免疫力、抗衰老等功能，广泛应用于各类心脏病、癌症、急慢性肝炎、帕金森症等疾病的治疗。本文对根瘤土壤杆菌（Agrobacterium tumefaciens KY-3085）发酵制备辅酶Q₁₀的发酵条件及代谢途径进行了研究。 本文以根瘤土壤杆菌为出发菌株，对发酵培养基组成进行了优化，优化培养基组成为：蔗糖4％、牛肉粉1％、蛋白胨0.5％、硫酸铵0.2％、K₂HPO₄ 0.05％、KH₂PO₄ 0.05％，MgSO₄·7H₂O 0.03％。并确定了种子液的最佳培养时间为24h和最佳发酵时间为120h。另外，还确定了最佳的发酵条件为：初始pH值为7.5、接种量为5％、装液量为60mL。 在对代谢途径分析的基础上，研究添加前体物质（对羟基苯甲酸、茄尼醇和CoQ₀）对辅酶Q₁₀合成的影响，并确定了最佳添加方式。结果表明，添加前体物质对辅酶Q₁₀含量均有提高。其中，在发酵48h添加0.6g／L茄尼醇时，辅酶Q₁₀含量最高为8.92mg／L，比不添加茄尼醇时提高了43％。本文还探讨了添加维生素B₁₂，甘油和甲硫氨酸对辅酶Q₁₀合成的影响。 由于CoQ₁₀是胞内产物，必须将细胞破壁，使之得以释放，才能进一步提取，故细胞破碎是提取胞内产物的关键步骤。考察了研磨法、有机溶更多还原

【Abstract】 The Coenzyme Q is a prenylated benzoquinone lipid, and it is also named ubiquinone. Members of the Coenzyme Q are found in microorganisms, plants, and the heart, kidney, liver of animals, where they function are redox carriers in mitochondria or in association with respiratory processes of electron transport chains. It is an antioxidant produced naturally in the cells. Coenzyme Q₁₀ exhibited excellent medical and physiological activities against various diseases such as heart diseases, cancer and so on.Microganisms are good source of CoQ homologs. In this paper, we studied the production and the metabolic regulation from Agrobacterium tumefaciens KY-3085.Through many experiments, the components of the fermentation medium were optimized. The optimum fermentation medium consisted of sucrose 4% , beef tea 1% , peptone 0.5%, （NH₄）₂SO₄ 0.2%, K₂HPO₄ 0.05%, KH₂PO₄ 0.05%, MgSO₄· 7H₂O 0.03%. The optimum cultivation time is 24h and the optimum fermentation time is 120h. Otherwise, the optimum fermentaion condition was assured, which was pH7.5, 0.5% amount of inoculation, 60 mL fermention culture medium.更多还原