【作者】 徐海红；

【导师】 张文； 金利通；

【作者基本信息】 华东师范大学 ， 分析化学， 2006， 硕士

【摘要】 高效液相色谱-电化学检测联用技术，作为分析化学的一个分支，由于其具有分离性能好、进样量少、灵敏度高等特点，已经成为化学、医学、生物、环境等领域的重要分析手段。近年来，随着生命科学的迅速发展，对生物样品信息的准确分析至关重要，但生物样品成份复杂，含量又往往很低，因此许多传统的、常规的分析方法与手段已不能满足这个领域研究的需要。如何实现在体、实时、连续的监测微量和超微量的生物样品，是一个迫切需要解决的问题，对于生物、医药乃至生命科学的发展都将具有重要的意义。 液相色谱—电化学检测技术正好适应了这种发展的需求，成为了检测生物样品的关键手段之一。但是，现有的液相色谱．电化学检测技术尚还存在着选择性和灵敏度等不尽人意的地方，因此，发展高性能的液相色谱-电化学检测技术，不仅对分析化学具有重要的意义，而且对生物学、医学以及整个生命科学的发展也具有重要的影响。本论文以新型液相色谱电化学检测器的研究开发为重点，研制了多种化学修饰电极用于液相色谱电化学检测器，提高了检测的灵敏度和选择性，将新型液相色谱-电化学检测与微渗析取样技术联用，建立了在生命体系中进行采样、分离和分析的新方法，为一些疾病的生理学、药物学及临床医学研究提供重要信息，同时对病理的研究和新药的开发也具有极为重要的意义。论文主要包括以下几个方面： 1．铂／聚甲基紫修饰电极—液相色谱电化学检测用于甲减患者血浆中同型半胱氨酸，半胱氨酸和甲硫氨酸的研究 本章对铂／聚甲基紫修饰电极的制备与应用进行了研究。循环伏安和液相色谱实验均表明铂／聚甲基紫修饰电极对同型半胱氨酸，半胱氨酸和甲硫氨酸具有良好的电催化氧化活性，在液相色谱电化学检测实验中，铂／聚甲基紫修饰电极对这些被测物的检测灵敏度比裸玻碳电极有显著的提高。将该修饰电极与高效液更多还原

【Abstract】 High performance liquid chromatography coupled with electrochemical detection (HPLC-ED) has become one of the most important approaches in the fields of chemical, biological, medical and environmental analysis since its appearance in the 1970’s for its high separation effectiveness, sensitivity and selectivity and its easiness of control. With the fast development of life science, accurate analysis of biological samples is the most crucial. However, many conventional analytical methods can’t match the requirements because of the complex component and low content of biological samples. How to realize the in vivo and on-line determination of biological micro-samples, which plays a critical role to the development of biology, medicine and to the life science, is an urgent problem.HPLC accommodates itself to this situation properly. However, the conventional HPLC-ED has its disadvantages, such as selectivity and sensitivity, etc. So, the development of high-quality HPLC-ED plays an important role not only in analytical chemistry progress but also in the advances of biology, medicine and even in the life science.In this dissertation, we laid our emphasis on the research of novel electrochemical detectors (ECD), and established some new methods of sample separation and analysis in life science by HPLC-ED coupled with microdialysis sampling technique. Some kinds of chemically modified electrodes (CME) which could enhance the sensitivity and selectivity in vivo analysis were developed and used as HPLC-ECD. These new analytical methods were successfully applied to the research on the diagnosis and treatment of some diseases such as hypothyreosis, Parkinson disease and acute appendix. The dissertation includes three chapters:更多还原