【作者】 康颖；

【导师】 曹建国；

【作者基本信息】 南华大学 ， 肿瘤学， 2006， 硕士

【摘要】 研究背景：前期研究表明，以白杨素(Chrysin，5，7-二羟基黄酮，ChR)为先导化合物，设计和合成的系列衍生物即7-二氟亚甲基-5-取代烷氧基黄酮对人胃癌细胞增殖活性具有抑制作用，其中7-二氟亚甲基-5-烯丙基黄酮(7-difluorinmethylene-5-allyl flavone，DFMeAlChR)作用最强。本实验旨在研究DFMeAlChR抑制体外培养人胃癌(SGC-7901)细胞系细胞生长和增殖作用及诱导凋亡作用，并初步探讨其作用机制。 方法：采用MTT比色法测定DFMeAlChR对体外培养SGC-7901细胞增殖活性的影响。用台盼蓝染色细胞计数法检测DFMeAlChR对SGC-7901细胞生长的影响。应用平皿克隆法观察DFMeAlChR对体外培养的SGC-7901细胞锚定依赖性生长的影响。AO染色荧光显微镜观察DFMeAlChR诱导SGC-7901细胞凋亡作用。PI染色流式细胞术(FCM)分析DFMeAlChR诱导SGC-7901细胞凋亡程度。采用western blot检测DFMeAlChR对SGC-7901细胞NF-κB、Bcl-2、Bax蛋白表达和活性的影响。 结果：MTT比色法测定结果显示，以浓度为0.1，0.3，1，3，10μM的DFMEAlChR处理SGC-7901细胞48小时后的增殖相对抑制率分别为3.1％，8.0％，17.6％，29.4％，39.0％；以浓度为1，3，10，30，100μM的ChR处理更多还原

【Abstract】 Background: Our previous studies showed that chrysin was taken as leader compound to design and synthesize a series of derivatives: 7-difluorinmethylene -5-substitutied-alkoxyl flavonoid compounds,which all had proliferation inhibitory effects of human gastric cancer cells . Among all the compounds,7-difluorinmethylene -5 -allyl flavone ( DFMeAlChR ) was the strongest .In this experiment , DFMeAlChR was taken as target compound to investigate the proliferation inhibitory effect of human gastric cancer cell line SGC-7901 in vitro and to explore its antitumor mechanism.Method: MTT assay was used to test the proliferation inhibitory effects of humangastric cancer cells (SGC-7901) by DFMeAlChR. Trypan blue staining assay was used to count the number of viability cells,to draw cell growth curve and to inverstigate the growth inhibitory effect of SGC-7901 cells by DFMeAlChR. Plate-colon assay was used to test colon formation inhibitory effect of SGC-7901 by DFMeAlChR .Acridine orange staining was used to observe apoptosis induced function of DFMeAlChR to SGC-7901 cells .PI staining flow cytometry (FCM) was used to analyse the apoptosis rate after treatment with DFMeAlChR for 48h .The protein level and activity of NF-κB,Bcl-2and Bax were examined by Western blot to explore the molecular mechanism of anti-gastric cancer action of DFMeAlChR .Result: The MTT assay showed that the proliferation inhibitory rate was 3.1%, 8.0%, 17.6%, 29.4%, 39.0%respectively after SGC-7901 cells treated with various更多还原