【作者】 唐湘宇；

【导师】 杨向东；

【作者基本信息】 南华大学 ， 病理学与病理生理学， 2006， 硕士

【摘要】 目前认为动脉粥样硬化疾病表现了一种高氧化应激状态，活性氧(reactiveoxygen species，ROS)诱导的氧化应激在动脉粥样硬化疾病的发病中有重要作用。在动脉粥样硬化形成和发展的病理过程，活性氧不仅氧化修饰低密度脂蛋白，促进泡沫细胞形成，而且活性氧介导的脂质过氧化还可以破坏血管内皮细胞，导致其功能障碍，从而促进动脉粥样硬化发生发展。在冠心病的发病过程中从初发脂纹到斑块损伤发展，内皮功能受损具有十分重要的意义。血管内皮细胞功能障碍是动脉粥样硬化的早期病理改变，而脂质过氧化则可能为内皮功能受损的始动环节之一。因此，抗氧化剂通过阻止氧化应激损伤对动脉粥样硬化疾病的防治是有积极意义的。 大量的流行病学资料显示，番茄红素的摄入量与冠心病的危险性呈负相关，血浆低番茄红素有更高的急性心肌梗死和脑卒中的危险性。但其防治这些疾病的具体机制尚不清楚。番茄红素是很好的抗氧化剂，通过阻断或抑制氧化应激损伤可能是番茄红素抗动脉粥样硬化的形成的一条途径。我们试图通过动物实验和细胞实验研究探讨其抗动脉粥样硬化疾病的作用及机制。 第一部分 番茄红素对兔动脉粥样硬化形成的影响 目的 观察番茄红素对高脂喂饲所致新西兰家兔动脉粥样硬化形成和脂质过氧化损伤的影响。 方法 采用高脂喂饲模型，将30只新西兰家兔随机分为3组：对照组、高脂模型组和番茄红素组，分别给予普通饲料和高脂饲料喂养，番茄红素组另加用番茄红素(每兔每天每公斤体重给予6％番茄红素粉剂60mg)；于实验开始前1天和开始后第2、4、6、8周末取空腹血测定血清总胆固醇，甘油三酯，高密度脂蛋白胆固醇，血清超氧化物歧化酶活性，丙二醛含量，血清一氧化氮含量和一氧更多还原

【Abstract】 There is now a consensus that atherosclerosis represents a state of heightened oxidative stress, Oxidative stress induced by ROS is considered to be an important part in the etiology of atherosclerosis. Dring the pathological course of forming and developing of atherosclerosis, ROS oxidate and modify the oxidated low density lipidprotein, and promote the formation of foam cells ;In addition, lipid peroxidation mediated by ROS can damage endothelium and results in endothelial dysfunction, which promote the initiating and developing of atherosclerosis. Endothelial dysfunction have an important role from the initiation of fatty streak to plaque lesion progress in atherosclerosis, it is the early pathological change of atherosclerosis, and lipid peroxidation injure may be one of the initiating factors of endothelium injure. Therefore, antioxidants should benefit to the prevention and treatment of atherosclerosis by holding back oxidative stress damages.Numerous epidemiologic studies have shown that intake of lycopene from tomato products was inversely related to the risky of coronary heart diseases, and low serum levels of lycopene were associated with an increased risk of myocardial infarction and stroke. However little is known about its mechanisms of prevention and treatment of CAD. Lycopene was a good antioxidant, prevention of oxidative stress damages may be a access to preventing atherosclerosis. We try to explore the effect and mechanism of preventing atherosclerosis of lycopene in animals experiment and cells experiment.Part Ⅰ the Effect of Lycopene on the Forming of RabbitsAtherosclerosisAim To observe the effects of lycopene on atherosclerosis and lipid peroxidation injury in New Zealand rabbits fed with cholesterol rich diet.Methods 30 New Zealand rabbits were randomly divided into three groups. They were individually housed in metal cages.Throughout the experimental period , they更多还原