【作者】 孙玉慧；

【导师】 杨永宗；

【作者基本信息】 南华大学 ， 病理学与病理生理学， 2006， 硕士

【摘要】 目的 肥大细胞（mast cells，MCs）是一种参与炎症及免疫应答反应的细胞。近年来随着对动脉粥样硬化（atherosclerosis，AS）认识的不断深入，发现MCs与AS发生发展的各个阶段都密切相关。AS斑块的细胞成分包括一些炎症细胞（T淋巴细胞，巨噬细胞，MCs等）和从血管中膜迁移到内膜的平滑肌细胞，但是关于MCs与平滑肌细胞的关系并不明确，本实验以ApoE^-/-小鼠为研究对象，复制AS模型，以Compound 48／80和富马酸酮替芬作为处理因素，研究Compound48／80和富马酸酮替芬对ApoE^-/-小鼠AS斑块的影响；同时以大鼠主动脉血管平滑肌细胞（vascular smooth muscle cells，VSMCs）为研究对象，以MCs作为处理因素，研究MCs对VSMCs迁移的作用，并初步探讨其作用机制。 方法 30只8周龄雄性ApoE^-/-小鼠随机分为3组：对照组，Compound48／80组和富马酸酮替芬组。对照组给予普通饲料饲养24周：Compound 48／80组给予0.15mg／kg Compound 48／80，每周两次腹腔注射，普通饲料饲养24周；富马酸酮替芬组：0.1ml／只，每天灌胃一次，更多还原

【Abstract】 Objective:Mast cells （MCs） are a kind of inflammatory-immune cells. Recently, with the progress of atherosclerosis（AS） etiology, we found that MCs were involved in the every evolutive phase of atherosclerosis. Vascular smooth muscle cells （VSMCs）, migrating from the media to the intima, and some inflammatory-immune cells（T lymphocyte, macrophage, mast cells and so on） are the cellar components of as lesions. But the relationship between MCs and VSMCs has not been clarified. Our study used ApoE^-/-mice as the animal model to observe the effects of Compound 48/80 and Ketotifen Fumarate on the lesions of AS, and used the cultured rats VSMCs and the compound 48/80 to clarify the effects of MCs on the movement of VSMCs. And we also discussed the mechanisms of VSMC’ s migration primarily.Methods:Thirty ApoE^-/- mice were randomly divided into three groups.There was the control group,the Compound 48/80 group and the Ketotifen Fumarate group. The Compound 48/80 group was intraparentoneal injected 0.15mg/kg Compound 48/80, and the frequency was two times every week; The Ketotifen Fumarate group was given 0.1ml per mouse by Gastric Perfusion, and the frequency was once a day. All the groups were fed by standard laboratory chow for 24 weeks, then all of the animals were euthanized. Blood was sampled from the eye. The serum lipids were Enzymatically detected. Sudan IV was used to stain the thoracic and abdominalis aorta. The vessel was fixed by in situ liquid flow. Thoracic aorta was fixed in 10% formalin, then embedded in paraffin .Serial sections and HE staining were carried out.更多还原