【作者】 韩丽娟；

【导师】 李贵荣；

【作者基本信息】 南华大学 ， 卫生毒理学， 2006， 硕士

【摘要】 第一章： 为建立环境样品中痕量甲醛检测的新方法，筛选出测定水中痕量甲醛的催化反应体系：HCHO-KBrO₃-AO-H₂SO₄。详细研究了该体系的吸收光谱特征。分别考察了溶液酸度、试剂用量、反应温度、反应时间和共存离子等影响因素，优化了适宜的反应条件，确定了吸光度值与甲醛浓度的线性关系。从而建立了测定甲醛的新的催化动力学光度法。 试验结果表明，在H₂SO₄介质中，HCHO-KBrO₃-AO体系的最大吸收波长为490nm，吸光度与AO的浓度符合Beer定律。一定条件下，甲醛对溴酸钾氧化吖啶橙的反应具有显著的催化作用，使其吸光度降低。吸光度改变值（ΔA）与甲醛浓度(C_HCHO)在0.0250～0.200μg／ml范围内呈良好的线性关系。方法的检出限为1.50×10^-2μg／ml。该方法操作简便，具有较高的灵敏度和良好的选择性。实验中该法应用于几种环境水样中甲醛的测定，相对标准偏差为1.23％～2.18％，样品加标回收率为93.4％～103.5％，结果满意。 第二章： 应用荧光光谱法，筛选出两种测定环境样品中痕量甲醛的催化荧光分析新体系：HCHO-KBrO₃-ST-H₂SO₄、HCHO-KBrO₃-PY-H₂SO₄。分别研究了两种体系的荧光光谱特征。分别考察了溶液酸度、试剂用量、反应温度、反应时间和共存离子等因素对该体系荧光强度的影响，优化了反应条件，确定了荧光强度值（ΔF=F₀-F）与甲醛浓度的线性关系，从而建立了测定甲醛的新的催化荧光动力学更多还原

【Abstract】 Chapter 1In order to establish new methods for the determination of trace formaldehyde in environmental water by kinetic spectrophotometry method , a new reaction system of HCHO-KBrO₃-AO-H₂SO₄ was selected. The properties of the absorption spectrum of the system were studied. The affecting factors on the strength of absorption including acidity, dosage, temperature, reaction time, coexisting ions and so on, and the optimal conditions of the reactions in the system were investigated. A new method based on the linear relationship of the absorbency （ΔA = A₀ - A ） to the concentration of formaldehyde in solution was established .The results showed that there was the catalytic effect of formaldehyde on the oxidation reaction of acridine orange with potassium bromate in sulphuric acid medium. The relationship of the reduced absorbency to the concentration of formaldehyde was good linearity and the maximum absorption peak located at 490nm. Based on these, a new catalytic kinetic spectrophotometric method for the determination of formaldehyde was developed. The detection limit was 1.50×10²μg/ml and the linearity range were 0.0250⁰.200μg/ml for formaldehyde. The method was applied to the determination of trace formaldehyde of water samples in environment, with satisfactory results.The RSD was 1.23%².18% , the recovery rate was 93.4%¹03.5% .Chapter 2In order to establish new methods for the determination of trace formaldehyde更多还原