【作者】 王淑瑞；

【导师】 齐浩；

【作者基本信息】 陕西师范大学 ， 动物学， 2006， 硕士

【摘要】 目的：研究磁场与抗肿瘤药物对肿瘤细胞的协同杀伤作用，并且建立B16黑色素移植瘤模型，研究磁场对在体B16黑色素瘤的影响。 方法： 1．采用MTT法检测了抗肿瘤药物(紫杉醇)和磁场对K562细胞(人白血病细胞)的增殖的影响。2．通过倒置显微镜、原子力显微镜、单细胞凝胶电泳方法及流式细胞仪分析检测技术进行磁场结合抗肿瘤药物对K562细胞协同杀伤效应的研究。3．采用MTT法检测静磁场或／和阿霉素对B16黑色素瘤细胞的影响。4．B16黑色素瘤移植模型的重建，并调查了静磁场对在体B16黑色素瘤的影响。 结果： 1．(1)K562细胞以1×10~5cells／mL的细胞密度接种，MTT检测的结果表明，磁场结合紫杉醇处理K562细胞时，磁场同10ng／mL紫杉醇处理K562细胞24h后，可以增加紫杉醇的抗肿瘤效应。 2．10ng／mL紫杉醇联合磁场处理K562细胞24h后，细胞形态改变，细胞膜不完整，细胞表面出现突起、孔洞；SCGE检测的结果表明，磁场与10ng／mL的紫杉醇联合作用K562细胞24h后可以增加细胞DNA的损伤程度，具有协同杀伤作用；检测细胞周期分布发现，紫杉醇结合磁场处理细胞24h后，大部分细胞被阻滞于S期与G2／M期，并且出现凋亡峰(亚二倍峰)。 3．B16细胞以5×10~4cells／mL的细胞密度接种，经静磁场处理，MTT检测的结果表明曝磁24h时细胞增殖即受到抑制(p＜0.01)；2ng／mL阿霉素联合磁场处理B16细胞24h后，MTT检测发现细胞活性显著降低，与单纯阿霉素组相比有极显著差异(p＜0.01)，SCGE检测的结果表明可以增加细胞DNA的损伤程度，具有协同杀伤作用。 4．B16黑色素瘤肿瘤移植模型的重建结果表明，细胞接种数量不同的小鼠均有肿瘤生长，致瘤率为100％；随着细胞接种数量的降低，小鼠肿瘤出现的时间逐渐延长，组间差异极显著(p＜0.01)；各组之间小鼠的存活时间随着细胞接种数量的降低而延长，组间差异极显著(p＜0.01)；不同接种数量的动物之间荷瘤时间没有差异(p＞0.05)；在体肿瘤生长状况为低剂量组肿瘤出现时间最晚，但肿瘤生更多还原

【Abstract】 Aim: The synergistic anticancer effects of magnetic fields with anticancer drug on cancer cells were studied. We reestablished B16 melanoma model hi mice and studied effects of static magnetic fields on B16 melanoma.Methods: 1. The influence of SMF or/and anticancer drugs (Taxol) on K562 cells were detected by MTT test. 2. The morphologic changes of K562 cells exposed, under SMF with anticancer drug were observed by microscopy, atomic force microscopy (AFM); The degree of DNA damage and the cell cycle distribution of K562 cells exposed under anticancer drugs or/and SMF were measured by Single cell gel electrophoresis (SCGE) and flow cytometer (FCM) . 3. The influence of SMF or/and adriamycin on B16 melanoma cells were detected by MTT test. 4. B16 melanoma model in mice were reestablished. And the effects of static magnetic fields on B16 melanoma were studied.Results: 1. When cell desity was l×l0~5cells/mL, SMF can enhance the anti-cancer effect of taxol after exposing under SMF and taxol (10ng/mL) for 24h.2. When K562 cells were treated with SMF and taxol for 24h, there were some changes in the morphology of K562 cells, such as some protuberances and holes-like structures appeared on the cell membrane; the degree of DNA damage were enhanced, and the cell cycle distributions were changed. K562 cells mostly were blocked S and G2/M phase.3. When cell desity was 5×10~4cells/mL, the growth of K562 cells were inhibited after exposing under SMF for 24h (p<0.01); When B16 cells were treated with SMF and adriamycin (2ng/mL) for 24h, the cell activities were significantly reduced( P＜0.01) and the degree of DNA damage were enhanced (p<0.01) .4. To study the effects of different number of tumor cells on tumor growth in mice. Different numbers of tumor cells were injected subcutaneously at the right armpit of更多还原