【作者】 王丽影；

【导师】 王喜军；

【作者基本信息】 黑龙江中医药大学 ， 生药学， 2006， 硕士

【摘要】 熊胆(Bear Bile)为熊科动物黑熊Selenarctos thibetanus Cuvier或棕熊Ursus arctos Linnaeus的干燥胆囊。味苦,性寒。具有清热解毒,凉心平肝,利胆明目等功效。熊胆粉为熊科动物黑熊经胆囊手术引流胆汁而得的干燥品,属国家卫生部批准的一类新药,临床上作为熊胆药物使用。 本文对熊胆及其替代品的研究情况进行了综述,在此基础上,首次建立了熊胆粉药材的色谱指纹图谱,并建立了以熊胆粉的主要有效成分牛磺熊去氧胆酸(Taurocherodeoxycholic acid)和牛磺鹅去氧胆酸(Tauroursodeoxycholic acid)为指标的含量测定方法。用此法对黑宝一个月份内连续10个批号和12个月份产熊胆粉以及9个不同产地的熊胆粉的药材样品进行质量评价,从而建立一种较好的控制药材内在质量的方法。 通过比较黑宝一个月份内连续10个批号和12个月份产熊胆粉药材指纹图谱可看出,黑宝不同的熊胆粉药材指纹图谱相似度较高,绝大多数大于0.9,说明黑宝熊胆粉总体的化学组成一致性较好,质量稳定,可作为黑宝熊胆粉的专属性指纹图谱;而从9个不同产地的熊胆粉指纹图谱可以看出,各地的熊胆粉相似度较差,由色谱工作站辅助自动进行峰匹配,仅能确定7个共有峰,较黑宝熊胆粉的色谱峰要少很多。该结果提示,各产地的熊胆粉较黑宝熊胆粉有些偏离,质量差异较大,因此在选择药材产地时应予以注意,缩小药材的产地范围,无疑对保证熊胆粉的质量一致具有重要作用,进而为熊胆粉药材质量控制提供参考。 对黑宝一个月份内连续10个批号和12个月份产熊胆粉两种主要有效成分同时定量的结果表明,黑宝熊胆粉单一成分含量或两种成分含量之和均较高,质量较好;一个月份内连续10个批号的熊胆粉相似度较好,质量均一稳定。而9个不同产地的熊胆粉质量差异较大,且两成分的含量较黑宝熊胆粉低,偏离较大。更多还原

【Abstract】 Bear bile is the drainage gallbladder of Selenarctos thibetanus Cuvier or Ursus arctos Linnaeus belonging to the family of bear animal. It possesses the efficacy for clearing heat and detoxifying, cooling heart and pacifying liver, bebefitting bile and brighting eye etc.. Bear bile power, the drainage bile drawing from the Selenarctos thibetanus Cuvier by operation, usually substitutes for bear bile in clinic.On the base of summarize, it is the first time to build up the fingerprint chromatography of bear bile power and to determine the mainly content of Taurocherodeoxycholic acid and Tauroursodeoxycholic acid. The evaluation method of quality was established .By comparison of fingerprint chromatography from Heibao successive 10 lot number in a month and 12 months bear bile power, it was proved that fingerprint chromatography of bear bile power from Heibao were basically similar. Similarity computing showed that most of the similarity reached above 0.9, proved that Heibao bear bile power content consistently and quality steady. The chromatography can be used as a specialized quality control for Heibao bear bile power. But by comparison of fingerprint chromatography from 9 producing areas, similarity computing showed that similarity not very well. Only matched 7 commom peaks much less than Heibao, the quanlity differed greatly. The results showed that it was necessary to contract the herb producing area, to control the quality for bear bile power.Through determination of two mainly ingredients of bear bile power, it was found that the content of Taurocherodeoxycholic acid and Tauroursodeoxycholic acid from Heibao were evidently higher than those other producing areas. The quality of Heibao bear bile power is better than the other 8.更多还原