【作者】 王丽；

【导师】 王亚贤；

【作者基本信息】 黑龙江中医药大学 ， 中西医结合基础， 2006， 硕士

【摘要】 系统性红斑狼疮(systemic lupus erythematosus.SLE)是自身免疫介导的,以免疫性炎症为突出表现的弥漫性结缔组织病。其特征为T,B细胞的异常活化、补体被激活及细胞因子网络失衡等,结果导致体内大量致病性自身抗体和免疫复合物的产生,临床出现各个器官、系统受累的症状。SLE目前尚无特异的治疗方法,鉴于当前治疗SLE的一线药物副作用较大,生物制剂疗法和传统中医药疗法备受关注。随着近年来对SLE病因病机的深入研究和祖国医药学的发展,本课题组取20-22g,8-10周龄,雌性BALB/c小鼠75只,随机分5组,即空白组、模型组、中药组(祛毒胶囊组)、西药组(强的松组)、联合用药组(祛毒胶囊与强的松联合用药组)各15只,参照了WHO免疫研究教学中心Tito Cavallo、Francisco报道的造模方法,i.p LPS诱导SLE样小鼠,给药15天,通过外周血IL—6和IFN-γ水平的测定以及肝脏和肾脏的病理观察,试图从免疫学、组织学角度透视中药祛毒胶囊治疗LPS诱导的狼疮样小鼠的机理和疗效,对祛毒胶囊的治疗作用进行深入研究。 研究结果显示:注射LPS后小鼠的外观状态和病理形态学改变符合SLE的诊断标准,该模型制备成功;祛毒胶囊是我校附属医院用以治疗SLE的验方,主要由秦艽、紫草、生地、生黄芪、红藤、雷公藤、升麻、丹参、生甘草等组成。本研究证明该药能有效降低致炎性细胞因子IL-6和IFN-γ的高表达,改善模型鼠的病理形态学变化。实验结果表明,祛毒胶囊具有一定的免疫抑制作用,在SLE的治疗中有一定疗效,但其确切作用机制有待于进一步深入研究。更多还原

【Abstract】 Systemic lupus erythematosus is a connective tissue disease which is mediated by autoimmunity with the characteristic symptom of immunity inflammation. Its character is abnormal activation of T cell and B cell, activation of complement and unbalance of cytokine network and so on, which result in production of massive pathogenic autoimmune antibody and immune complex in the body. The clinical representation is involved in symptom of each organ and system. At present, there is no specific cure about SLE and because . As the side effect of the first line drug is severe in SLE treatment now, biological therapy and traditional Chinese medicine therapy are highly focused. With the deep study of SLE pathogeny and the development of traditional Chinese medicine in the recent years, we allocate 75 female BALB/ c mice of 20-22g, 8-10 week-age to 5 groups randomized, i.e. blank group、model group、 traditional Chinese medicine group (Qu Du capsule group)、 Western medicine group (prednisone group)、 drug combination group (drug combination of Qu Du capsule and prednisone group), every group having 15 mice. We induce SLE mousse with injecting LPS peritoneal accordance with the method of making model which is reported by Tito Cavallo、 Francisco in WHO Immunology Research and Training Centre and dosage 15 days .Through detecting IL — 6 and IFN-y of Peripheral blood and observing pathology consequence of liver and kidney, we try to observe mechanism and curative effect of Qu Du capsule which is used to cure SLE mice with LPS induction in the aspect of immunology and histology and study therapeutical effect of Qu Du capsule deeply.The study consequence is followed. The appearance of rats injected LPS and the altering of pathologic morphology come up tothe diagnostic standard of SLE. The model is prepared successfully;Qu Du capsule is an effective medical prescription of curing SLE in the affiliated hospital of Heilongjiang university of Chinese medicine and it is composed of Qinjiao> Zicao^ Shengdk Huangqi> HongtengN Leigongteng> Shengii^ Danshen> Shenggancao and so on. This study proofs that Qu Du capsule can decrease cytokine of leading to inflammation —IL-6 and IFN-y expression effectively and improve the altering of pathologic morphology of model. The experimental result represents that Qu Du capsule have a given immunological inhibitor function and a given cure in the therapy of SLE. But its definite mechanism of action will be study more deeply.更多还原