河北道地药材指纹图谱研究

【作者】 张志斐；

【导师】 张兰桐；

【作者基本信息】 河北医科大学 ， 药物分析学， 2006， 硕士

【摘要】 知母为百合科(Liliaceae)植物知母Anemarrhena asphodeloides Bge.的干燥根茎,其性味苦、寒,具有滋阴降火、润燥滑肠的功效。知母根茎中含大量甾体皂苷,现代药理研究表明知母皂苷及皂苷元为其主要活性成分。中药化学成分的多样性与复杂性是其发挥疗效的物质基础,而传统的中药材质量评价方法大多以单一或少数几个成分做指标进行质量评价,不能全面反映其质量情况。中药指纹图谱正是运用现代分析技术对中药化学信息以图形(图像)的方式进行表征并加以描述的中药质量分析的一种新方法。它通过反映中药内在化学成分的种类和数量,提供全面的、综合的信息以控制中药材及中药制剂的质量,是目前国际上能认可的、较好地反映了中药材及中药制剂质量的质量控制方法。因此,建立道地药材知母的指纹图谱对全面控制知母药材的质量是十分必要的。目的:建立河北道地药材知母的HPLC指纹图谱,获得对照图谱,并与不同产地知母药材指纹特征相比较,为科学评价与有效控制知母药材质量提供新方法。方法:(1)提取:比较了不同提取方法、提取溶剂及提取时间的提取效果,选择提取成分较多,提取率较高的提取条件。(2)色谱条件:选用合适的色谱柱及检测器,调整流更多还原

【Abstract】 Rhizoma Anemarrhenae is the dry root and stem of Anemarrhena asphodeloides Bge, which belongs to the family of Liliaceae. It is reported that the dry root and stem has the quality of bitter and cold,which can nourish yin, clear away heat evil, moisturize dryness and promote bowel movement. There are plenty of Steroid saponin in the root and stem of Anemarrhena asphodeloides Bge. It has showed in modern pharmacology that saponin and aglycone are bioactive components in Rhizoma Anemarrhenae.Variety and complexity of chemistry components of TCM are the physical foundation to produce curative action. However, one or several components are always only used in quality evaluation of TCM as index, which always can’t reflect its internal quality comprehensively. The modern analytical technique is applied to study fingerprints by using figures to describe the internal chemical information of TCM. In this case, fingerprints just are a new method to reflect the differences of description and quantity of chemical information. It can be used更多还原