【作者】 孙艳玲；

【导师】 张炳华；

【作者基本信息】 新疆医科大学 ， 免疫学， 2006， 硕士

【摘要】 目的：以鼠伤寒沙门菌(Salmonella typhimurium，STM)感染小鼠为模型，研究两岐双岐杆菌(Bifidobacterim bifidum，B．bifidum)在体内对STM感染小鼠的治疗作用。方法：先用硫酸链霉素给正常小鼠灌胃2d，出现菌群失调症状，再用STM灌胃造成小鼠STM感染，使用两岐双岐杆菌液干预治疗STM感染小鼠，检测不同治疗时间各组小鼠粪便STM菌落数、肝脾指数、血及脾中TNF-a的含量。采用SPSS12.0统计软件包进行数据处理分析，统计学方法采用双因素重复测量资料的方差分析、单因素随机化设计的方差分析、卡方检验、多组均数比较的非参数秩和检验。结果：随着治疗天数的增加，STM菌落数逐渐减少，未干预组、生理盐水组及双歧杆菌组比较结果均为P＜0.01；组间比较，双歧杆菌组STM数下降最快。未干预组、生理盐水组及双歧杆菌组肝脾指数比较均P＞0.05；STM感染各组与正常组血及脾中TNF-a的含量比较均P＜0.05；STM感染各组中未干预组、生理盐水组及双歧杆菌组血中TNF-a的含量比较P＞0.05，脾中TNF-a的含量比较P＜0.05；组间比较，未干预组与双歧杆菌组比较，P＜0.05。结论：随着治疗天数的增加，STM菌落数逐渐减少，未干预组、生理盐水组及双歧杆菌组比较有统计学意义，提示双歧杆菌对小鼠STM感染有治疗作用；根据肝脾指数的比较结果，说明其对免疫器官干预治疗作用不够显著；正常组、未干预组、生理盐水组及双歧杆菌组血及脾TNF-a含量比较表明，STM感染各组血及脾中TNF-a含量均高于正常组，且双歧杆菌组最高，提示双歧杆菌干预对感染机体免疫细胞分泌TNF-a有一定促进作用。更多还原

【Abstract】 Objective:The purpose of this study was to examine whether bifidobacteria have the treatmental effect oh experimental salmonellosis in mice or not.Method:Firstly, normal mice was given orally by SM(qd×2d), and the dysbacteriosis were caused in them ,and then they were given orally by STM,these mice were formed experimental salmonellosis models ,finally these salmonellosis models were treated by bifidobacterium ,and count respectively the cfu/g of STM number in the mice excrement during different time of therapy,detect the index numbers of liver and spleen respectively, detect content of TNF-a in blood and in spleen respectively. Apply statistical package of SPSS 12.0 to carry out data processing analysis.and adopt statistical method of Repeated Measures of General Linear Model ,One-way ANOVA of compare means,Chi square test,K Independent Sample Test of Nonparametric Test, ANOVA of Orthogonal Experiment. Result: The CFU of STM decreased gradually by the increasing day of the treatment, on Statistics,there were remarkable changes of the CFU of STM among model groups (P<0.01); group comparison,the most decreasing is the group of bifidobacteria,there were no changes of the index number of liver and the index number of spleen among model groups(P>0.05); The content of TNF-a in blood and spleen in model groups are higher than in normal group;there were no changes of content of TNF-a in blood among model groups(P>0.05); there were changes of content of TNF-a in spleen among model groups(P<0.05); group comparison,the group of bifidobacteria is higher更多还原