【作者】 刘杰；

【导师】 于卓； 金黎平；

【作者基本信息】 内蒙古农业大学 ， 作物遗传育种， 2006， 硕士

【摘要】 本试验以二倍体马铃薯为材料,通过对其炸片性状的多年鉴定,筛选优良育种材料,并构建一张分子连锁图谱,为分子标记辅助育种及目标基因或主效QTL的定位克隆奠定良好的基础。主要结果如下:1.试验筛选出9份炸片颜色好的二倍体材料,其编号分别为02018-32、02018-44、02018-93、02018-99、02018-105、02018-179、02018-221、02018-242和02018-248。2.筛选出5份耐低温储藏且综合加工品质较好的二倍体育种材料02018-93、02018-94、02018-105、02018-176和02018-248;同时发现,02018群体的材料贮藏近3个月,在6℃条件下要比4℃下更容易通过回暖使其炸片颜色变好。3.利用许多重要性状都存在显著差异的二倍体马铃薯亲本08675-21、09901-01及其125份F1分离单株系,构建了国内第一张马铃薯AFLP分子连锁图谱。其中母本图谱含有75个标记,覆盖基因组全长512cM,平均图距为6.83cM;父本图谱含有95个标记,覆盖基因组全长578cM,平均图距为6.08cM。更多还原

【Abstract】 Potato clones with desirable chipping traits were breeded and selected at diploid level, as well as to construct a molecular linkage maps based on AFLP technology by using a diploid segregating population, so that the chipping traits could be improved by the molecular marker-assisted selection. Furthermore, it would be ready for mapping and isolation of the genes or main effect QTLs about chipping traits. The results were as follows:1. 9 clones of diploid potato with light chip colour were screened out after the evaluation during 2003～2005, and are 02018-32、02018-44、02018-93、02018-99、02018-105、02018-179、02018-221、02018-242 and 02018-248.2. 5 diploid clones of 02018-93、02018-94、02018-105、02018-176 and 02018-248 with good cold chipping qualities were obtained after the two-year evalution under 4℃/6℃cold-storage and reconditioning at 18℃. The experiment results also showed that tubers of clones from 02018 population stored at 6℃for 80 days were much easier to be reconditioned than those-stored at 4℃for 90 days.3. A molecular linkage map of potato (Solanum tuberosum L.) was first developed in China based on amplified fragment length polymorphism (AFLP) markers by using a F1 segregating population from the cross between the diploid potato parents of 08675-21 and 09901-01. The genetic map included 24 main linkage groups, and the female-maps covered 512cM with an average distance of 6.83cM between adjacent markers while the male-maps cover 578cM with an average distance of 6.08cM between adjacent markers.更多还原