【作者】 赵桂梅；

【导师】 张丽萍；

【作者基本信息】 东北师范大学 ， 生物化学与分子生物学， 2005， 硕士

【摘要】 灵芝孢子粉经石油醚脱脂,70%甲醇回流脱去单寡糖后,经热水提取由乙醇醇析得到粗多糖GLP,经气相色谱测定粗多糖由葡萄糖和半乳糖组成,其摩尔比为14.90:1.00。GLP经冻融分级及Sepharose CL-6B柱层析得到GLP2和GLP3。经Sepharose CL-6B柱层析、DEAE阴离子交换层析检查,均呈单一狭窄对称峰,进一步经HPLC检验,GLP2和GLP3的图谱均呈单一谱带,表明GLP2和GLP3在分子量大小和极性上都较为均一,分子量分别为5.37×104和1.41×104。气相色谱表明二者均由葡萄糖和半乳糖组成,其摩尔比分别为12.31:1.00和4.46:1.00。GLP2和GLP3的结构分析采用了部分酸水解、高碘酸氧化、Smith降解、甲基化分析及IR、GC、GC-MS和13C-NMR等方法。分析结果表明:GLP2为少分支结构,主要由β型糖苷键构成,由1→3连接和1→6连接的葡萄糖构成主链,部分1→6连接葡萄糖在3位或4位有分支,平均每十个己糖基有2个分支,侧链为1→4连接的半乳糖,分支末端残基为葡萄糖。GLP3为少分支结构,主要由β型糖苷键构成,其基本结构中主链由1→3连接的葡萄糖和1→6连接的半乳糖构成,部分1→3连接的葡萄糖在6位有分支,部分1→6连接的半乳糖在2位或4位有分支,平均每十个己糖基有2个分支,1→4连接的半乳糖或1→6连接的葡萄糖位于侧链,分支末端残基为葡萄糖。更多还原

【Abstract】 The spores of Ganoderma lucidum were previously defatted with petroleum ether andgot rid of monosaccharides and oligosaccharides with 70% methanol. Then the residue wasdecocted with hot water. The water soluble crude polysaccharide GLP was extracted from theresulting aqueous mixture by the method of ethanol precipitation. G.C. analysis illustrated thatGLP consisted of Glc and Gal in the molar ratio of 14.9:1.00.GLP was purified by freeze thawing and Sepharose CL-6B gel filitration. Two fractionswere obtained: GLP2 and GLP3. The Sepharose CL-6B gel filitration,DEAE-Sephadex A-25and HPLC analysis indicated that GLP2 and GLP3 were both homogeneous polysaccharideson molecular weight and polarity because of their single strait symmetry apex. Their molecularweight is 5.37×104 and 1.41×104 respectively. GLP2 and GLP3 are both composed of Glc andGal. The molar ratio is 12.31:1.00 and 4.46:1.00 respectively.By means of partial hydrolysis with acid, periodate oxidation, Smith degradation,methylation, IR, GC, GC-MS and 13C-NMR, the structures of GLP2 and GLP3 were identified.GLP2 is mainly made up of β configuration glycosidic bond and is a lowly branchedmolecule with basic structure composed of (1→3)-linked-Glc and (1→6)-linked-Glc residuesin the main chain. Some of (1→6)-linked-Glc residues are substituted at 3-O or 4-O. Onaverage, there are 2 branches among 10 units. The side chain is composed of(1→4)-linked-Gal. The nonreduced end is Glc.GLP3 is mainly made up of β configuration glycosidic bond and is a lowly branchedmolecule also. The main chain is made up of (1→3)-linked-Glc and(1→6)-linked-Gal.Some of (1→3)-linked-Glc residues are substituted at 6-O and some of (1→6)-linked-Galare substituted at 2-O and 4-O. On average, there are 2 branches among 10 units. The(1→4)-linked-Gal and (1→6)-linked-Glc are on the side chain. The nonreduced end is Glc.更多还原