【作者】 奚玉石；

【导师】 张守勤；

【作者基本信息】 吉林大学 ， 农业机械化工程， 2006， 硕士

【摘要】 本论文在分析了山楂叶总黄酮传统提取分离方法基础上,利用超高压提取技术提取山楂叶总黄酮。通过单因素试验,对每个影响因素进行详细的研究,在此基础上采用正交试验法对提取工艺进一步优化,得到超高压提取最优操作工艺参数;通过山楂叶总黄酮超高压提取与其它方法提取的对比试验,进一步研究和分析超高压提取方法的特点,为超高压提取山楂叶总黄酮的工业化生产提供理论和试验依据。对大孔树脂分离纯化山楂叶总黄酮工艺进行详细研究。试验结果表明,采用D101大孔树脂分离纯化山楂叶总黄酮是可行的。并且其分离纯化工艺结果满足工业生产要求,为山楂叶总黄酮提取分离的工业化生产提取了新的方法。对山楂叶提取物抗氧化活性进行简单的考察,也表明山楂叶具有较强的抗氧化能力,应进一步研究可开发山楂叶黄酮食品抗氧化剂。更多还原

【Abstract】 The ultra-high pressure extraction(UPE) is a new extraction method of theessential components of plant,and the low energy consumption, the short timeconsumption and the quality of selective extraction is its major characteristic.In recentyears ,more and more scholars research the UPE in the natural medicine extractionfield. The traditional extraction methods of flavoiods from the leaves of CrataegusPinnatifida Bge.have much disadvantage such as numerous and diverse craft, the longtime and the low rate. Consequently, It is necessary for us to study extractionionmethods in search of the best extraction method of flavoniods from the leaves ofCrataegus Pinnatifida Bge.This article researched amply UPE of flavoniods fromCrataegus Pinnatifida Bge;The pharmaceutical factory discovered thatnewdosage-form of flavanoiods of the leaves of Crataegus Pinnatifida Bge. Can appearthe sedimentation after a period of depositing because macro-molecule impurity hasnot been able to clean out completely.So it is significative to research thoroughlyseparation craft parameter of total flavanods of the leaves of Crataegus PinnatifidaBge. In this paper the main contents of research and results are as follow: 1. It is the first time to extract flavoniods from the leaves of CrataegusPinnatifida Bge. by the UPE of at room temperature. Based on the study of the effectsof pressureSolvent concentration, ratio of solvent to feed material, extraction duration and extraction times on the extraction yield of flavonoids ,the optimized extraction conditions were determined by the orthogonal test design as follows:extraction solvent: 50 %ethanol;ratio of solvent(ml) to feed material(g): 45;extraction duration:10s,extraction pressure:300MPa;and 9.15% of extraction yield of flavoniods from theleaves of crataegus pinnatifida Bge was obtained.2. Comparison of UPE with other extraction method such as heat reflux,ultrasonic-assisted etc is studied, every method is operated according itself optimumconditions. The results indicate that the ultra-high pressure extraction could be cardedout not only in the shorter time but also in the higher yield.and UPE is superior to theother traditional extraction methods on the extraction of flavoniods. During theexperiment we observed that the extraction liquid of UPE is the most stable and theleast impurity in appropriate craft parameter situation ,and the extraction solution ofother methods is black、green and turbid,which showed that UPE has the quality ofselective extraction, in appropriate craft parameter situation,the impurity protein,sugar, chlorophyll to be able to reduce, which also indicated that, UPE of flavoniodsfrom Crataegus Pinnatifida Bge. is propitious to purify total flavoniods.3. It take merely 3 minutes to Complete entire UPE. the effective componentextraction ratio and the extraction efficiency enhance.4. It is the first time to uses the macroporous adsorption resin to purify totalflavanods of the leaves of Crataegus Pinnatifida Bge. the optimized separationcondition were determined by the dynamic adsorption experiment and staticadsorption experiment as follows: the total flavanone saturated adsorption quantity ofthe D101 macroporous adsorption resin: 78mg/g;the water elute quantity : 8BV;theethanol elute quantity is 4BV;adsorption speed of flow is 3BV/h, desorption speed offlow is 2BV/h;the eluting sequence is that with the fuicl of 10% ethyl alcohol eluting,abandoned, the fluid of 70% ethyl alcohol elutes is collected, concentration anddryness, and total flavanods content may achieve above 80%.5. The effect of the different concentration ethanol as elution solvent onflavanods was studied. And the experimental results showed that the differentconcentration ethanol as elution solvent, the kind and content of the flavonoids areobviously different. If taking 2"-O-Rhamnosylvitexin as the goal, the eluting sequenceis that with the fluid of 30% alcohol eluteing abandoned, the fluid of 50% alcoholeluting is collected, concentration and dryness:6. The antioxidant ability of 7 kind of Chinese traditional medicine were studied.The research results indicated that the leaves of Crataegus Pinnatifida Bge take on thehigh antioxidant ability.At present the leaves of Crataegus Pinnatifida Bge are rich inchina, but few leaves of Crataegus Pinnatifida Bge are empoldered and utilized,therefore it’s significative to thoroughly research the leaves of Crataegus PinnatifidaBge.and its application.7. The flavonoids content in the leaves of Crataegus Pinnatifida Bge. Of differentgrowth stages are studied.The contents of flavonoids were determined by HPLC andUV-spectrophotometry. At different growth stages, the content of total flavonoids and2"-O-Rhamnosylvitexin were also the highest at August;afterwards, the flavonoidscontent decreased, so August was appropriate time in order to obtain flavonoidscomponents from the leaves of Crataegus Pinnatifida Bge. Its antioxidant ability alsopresents such tendency.更多还原