【作者】 王露；

【导师】 黄楠； 陈俊英；

【作者基本信息】 西南交通大学 ， 材料学， 2006， 硕士

【摘要】 本文采用非平衡磁控溅射沉积技术,制备了具有金红石结构的Ti-O膜,并在此基础上,通过表面生物化方法之一的固定细胞外基质蛋白中的纤连蛋白对Ti-O膜进行表面改性。先通过盐酸和双氧水活化Ti-O膜表面使其产生活性的羟基基团,再涂覆上一层多聚赖氨酸(Poly-L-Lysine,PLL)作为中间过渡层,以促进生物大分子的粘附,最后在表面上固定纤连蛋白(Fibronectin,FN)。并采用傅立叶变换红外光谱仪(FTIR)、X射线光电子能谱(XPS)、原子力显微镜(AFM)、扫描电子显微镜(SEM)、接触角测量等方法对每一步处理后的样品表面特征进行了测试与分析,最后在样品表面进行了体外人脐静脉血管内皮细胞培养实验。重点研究了Ti-O膜表面固定纤连蛋白的表面改性方法以及对人脐静脉血管内皮细胞粘附及生长行为的影响,目的在于寻求能促进内皮细胞在Ti-O膜表面粘附和生长的表面生物化方法。 Ti-O膜经盐酸和双氧水活化后,FTIR结果显示表面可产生羟基基团,XPS中-OH对应的O峰位增强,接触角减小。 在活化的Ti-O膜表面涂覆PLL后,FTIR及XPS结果表明Ti-O膜表面的羟基可以和PLL中的羧基基团反应生成酯键,使PLL化学键合到Ti-O膜表面。SEM及AFM结果显示表面形貌发生改变,有尺寸在100nm左右的微小颗粒出现,接触角增加。 在涂覆了PLL的样品及Ti-O膜表面固定FN后,FTIR及XPS结果得出样品表面都有不同程度的吸附FN。接触角进一步增大。 人脐静脉血管内皮细胞体外培养实验结果表明,Ti-O膜具有一定的内皮细胞亲和性,FN对内皮细胞的生长有较好的促进作用,且在Ti-O膜表面通过PLL修饰后固定FN比直接固定FN能使细胞更好的粘附和生长。 综合分析结果表明,FN能促进内皮细胞的粘附和生长,通过表面活化后涂覆PLL并固定FN的方法能使内皮细胞在材料表面更好的粘附和生长。更多还原

【Abstract】 In this paper, the titanium oxide films with Rutile structure were synthesized by unbalanced magnetron sputtering and then modified by one surface biomodification method. The titanium oxide films were activated by HCL and H₂O₂ to produce hydroxyl group, then coated with poly-L-lysine to promote the adsorption of biomacromolecule and immobilized with fibronectin. The surface characteristics of samples were investigated using Fourier transfer infrared spectrum （FTIR）, X-ray photoelectron spectroscopy （XPS）, Atom force microscope （AFM） and Scanning electron microscope （SEM）. The wettability of Ti-0 films were studied by a contact angle method. The biological behavior of cultured human umbilical vein endothelial cells （HUVECs） onto different samples was evaluated by the in vitro HUVECs cultured experiment. This work focus on the surface modification of immobilization of fibronectin and its influence to the biological behavior of cultured HUVECs onto titanium-oxide films.The results of FTIR indicated that the titanium oxide film can produce hydroxyl group activated by HCL and H₂O₂. The peak of O element corresponding to hydroxyl group increased in results of XPS, the contact angle was lower.The results of FTIR and XPS indicated that the hydroxyl group can react with carboxyl of poly-L-lysine to make it bond chemically with film surface. The results of SEM and AFM indicated that the surface morphology changed with the appearance of little grains in size of 100nm and contact angle of the surface increased.After immobilized with fibronectin, the surface of titanium oxide film can adsorb fironectin which showed in the results of FTIR and XPS. The contactangle became larger.The in vitro HUVECs cultured investigation justified that the endothelial cells have affinity with Ti-0 film, fibronectin can promote the adhesion and growth of endothelial cells, the results of HUVECs cultured experiment of Ti-0 film which modified by poly-L-lysine and immobilized with fibronetcirr is better than that of Ti-0 film immobilized directly with fibronctin.All these results indicated that fibronetin can promote the adhesion and growth of endothelial cells, the method of coating with PLL and immobilizing with fibronectin can promote the adhesion and growth of endothelial cells. This method provided a surface biomodification way for improving the biocompatibility of Ti-0 film.更多还原