【作者】 李刚；

【导师】 刘君；

【作者基本信息】 青岛大学 ， 外科学， 2005， 硕士

【摘要】 目的 目前国内外对血小板活化的研究主要集中在内科性疾病如心脑血管疾病、糖尿病及一些炎症性疾病的相关研究上,但对下肢动脉硬化闭塞症(LEAO)伴原发性高血压(EH)老年患者血小板的活化状态特别是血小板微粒的研究较少。本研究通过对单纯EH患者、LEAO合并EH患者以及对照组血小板微粒膜糖蛋白和血小板聚集率的测定,探讨单纯EH以及LEAO合并EH老年患者血小板的活化状态。方法 采用病例对照设计,老年LEAO并EH组患者20例及单纯EH患者组20例、健康老年组20例,以单克隆抗体(McAb)为探针,用流式细胞术,对各组的血小板微粒糖蛋白PAC-1、表面选择素(CD62P)进行检测:同时观察血小板聚集率(PAgT)的变化。结果 (1).血小板微粒膜上PAC-1、CD62P的表达水平在单纯EH组和LEAO并EH组明显高于对照组;而LEAO并EH组的PAC-1、CD62P的表达又高于单纯的EH组。(2).血小板聚集率(PAgT)在单纯EH组和LEAO并EH组明显高于对照组,LEAO并EH组的PAgT高于单纯EH组,且LEAO并EH组其PAC-1、CD62P的表达与PAgT呈正相关(r=0.8701,0.7931,p<0.01)。结论 (1)血小板活化微粒标志物CD62p、PAC-1能更好地反应血小板的活化情况。(2)单纯EH组和LEAO并EH组中PAC-1、CD62P的表达和血小板聚集率都是增高的,尤其以合并LEAO的患者更为突出。提示下肢动脉硬化闭塞症并原发性高血压的发生发展与血小板活性的增高及血小板聚集功能的增强有关。更多还原

【Abstract】 Objective : At the present time, the research of the platelet activation is focused on thecorrelation in the internal medical diseases, such as cardiac or cerebrovascular diseases, diabetes mellitus and inflammatory diseases. But the research of the platelet activation, especially platelet microparticle , is rare in patients with arteriosclerosis obliterans of legs accompanied by essential hypertension(LEAO EH). To investigate the change of platelet activity in patients with LEAO EH and determine whether platelet is activiated in patiens with LEAO EH, we measured the expression of membrane glycoproteins(GP) on platelet microparticles and the level of platelet aggregation rate (PAgT) in patients with EH and with LEAO EH (n=20), the elder essential hypertensive patients (n=20) and healthyvolunteers(n=20) served as control. Methods: The platelet activation markers includingmicroparticle membrane glycoproteins of CD62p PAC-1 were fluorescence labeled with their corresponding monoclonal antibodies and analyzed by FCM, and the change of theplatelet aggregation rate (PAgT) was investigated together in all patients. Results : 1.Of three group, expression level of CD62p PAC-1 in EH group and LEAO EH group is increased. On the one hand, the expression of CD62P PAC-1 is higher in LEAO EH group than in pure EH group. 2. Platelet aggregation rates in patients of EH and LEAO EH are increased significantly;there was more increase in EH and LEAO EH group than that in pure EH group. Moreover, the expression level of CD62P PAC-1 was positively correlated with the platelet aggregation rate (r=0.8701, 0.7931, p<0.01)Conclusion: 1. CD62p PAC-1 , The platelet microparticle activation markers,reflected the change of platelet activity better. 2.The expression of CD62p PAC-1 and PAgT is higher in the pure EH group and LEAO EH group, and the increase of LEAO EH is more obvious. These results suggest that increase of platelet activity and platelet aggregation rate are related to occurrence of LEAO EH.更多还原