【作者】 崔洪艳；

【导师】 牛秀敏；

【作者基本信息】 天津医科大学 ， 围产医学， 2006， 硕士

【摘要】 目的:①探讨血管紧张素转换酶(ACE)基因插入/缺失(I/D)多态性与不同发病类型重度子痫前期的关系,以揭示早发型重度子痫前期的发病机制;②探讨ACE基因I/D多态性与重度子痫前期肾脏损害的关系,以揭示重度子痫前期肾脏损害的分子生物学机制。 方法 应用聚合酶链反应(PCR)技术检测63例重度子痫前期患者和40例正常孕妇的ACE基因I/D多态性分布。 结果 ①重度子痫前期组与正常孕妇组比较,基因型分布和等位基因频率均无显著性差异,(x~2值分别为2.1218;2.0019,P>0.05);②早发型重度子痫前期组与晚发型重度子痫前期组比较,基因型分布和等位基因频率均无显著性差异,(x~2值分别为1.8333;1.6805,P>0.05);③重度子痫前期肾损害组与无肾损害组及正常孕妇组比较,基因型分布和等位基因频率均有显著性差异,(x~2值分别为18.2952;16.6012,15.3279;13.3054,P<0.05);重度子痫前期无肾损害组与正常孕妇组比较,基因型分布和等位基因频率均无显著性差异,(U。值为0.0283;x~2值为0.2590,P>0.05)。 结论 ACE基因I/D多态性与重度子痫前期发病无密切相关。ACE基因I/D多态性与不同发病类型重度子痫前期的发病无密切相关,但与重度子痫前期肾脏损害相关,ACE基因DD型可能是重度子痫前期肾脏损害的独立危险因素。更多还原

【Abstract】 Objective: The aim of the study was (1)to investigate the relationship between the Insertion/Deletion Polymorphism of Angiotensin Converting Enzyme Gene and different kinds of severe preeclampsia(S-PE), so that to further find out the pathogenesis of early onset severe preeclampsia(EOSP);(2)to investigate the relationship between the Insertion/Deletion Polymorphism of Angiotensin Converting Enzyme Gene and kidney lesion, in order to find out the molecular mechanism of the kidney lesion resulting from severe preeclampsia. Methods: ACE genotyping with polymorase chain reaction(PCR) was performed in 63 patients with S-PE and 40 normal pregnant women. Results: (1) The frequencies of DD genotype and deletion allele showed no significant differences between patients with S-PE and normal pregnant women, x~2 were 2.1218, 2. 0019, respectively (P>0. 05). (2) The frequencies of DD genotype and deletion allele showed no significant differences between groups of EOSP and late onset severe preeclampsia(LOSP), x We 1. 8333, 1. 6805, respectively (P>0. 05). (3) The frequencies of DD genotype and deletion allele among S-PE complicated with kidney lesion were higher than those among S-PE without kidney lesion and normal pregnant women, x~2 were 18.2952, 16.6012;15.3209,13.3054 respectively (P<0. 05). The frequencies of DD genotype and deletion allele showed no significant differences between S-PE without kidney lesion and normal controls, U_C was 0.0283, x~2 was 0.2590(P>0.05). Conclusions:Although I/D polymorphism of ACE gene has no relationshipwith the type of severe preeclampsia, it has some relationship with kidney lesion. DD genotype of ACE gene may individually act as a high risk predict for S-PE kidney lesion.更多还原