【作者】 蔺海燕；

【导师】 许家军； 刘芳； 杨向群； 吴爱群；

【作者基本信息】 第二军医大学 ， 人体解剖与组织胚胎学， 2006， 硕士

【摘要】 本文通过建立大鼠视神经损伤模型,应用免疫组织化学、原位杂交组织化学、免疫荧光双标组织化学和基因芯片的方法从形态学、蛋白表达和基因表达的角度研究视神经损伤和/或移植自体预变性腓总神经后神经胶质细胞和神经再生的变化,旨在探讨受损视神经大胶质细胞去分化及诱导的可行性。结果发现视神经伤后巢蛋白表达升高,胶质细胞基因表达发生变化等;移植周围神经后巢蛋白表达进一步升高,并能影响胶质细胞基因表达等。观察到神经胶质细胞中巢蛋白分别与GFAP、MBP共存。结果提示视神经吸断后,其微环境不利于神经再生,胶质细胞基因表达发生变化,大胶质细胞呈去分化趋势;移植周围神经改善了视神经损伤后抑制性微环境,影响胶质细胞基因表达,并诱导大胶质细胞去分化。更多还原

【Abstract】 To explore the dedifferention and its induction in glial cells and nerve regeneration by autograft pre-degenerateded nerve, changes of morphology and expression of proteins and genes were studied by immunohistochemistry ABC method, in situ hybridization histochemistry, double immunofluoresence histochemistry and gene chip after optic nerve injury in rats. After optic nerve injury, expression of Nestin was up-regulated. Gene expression in macroglia cells changed and so on. Expression of Nestin was further up-regulated by autograft perpheral nerve. In addition, autograft peripheral nerve regulated expression of a lot of genes in macroglia cells. These results suggest that there be not a successful regeneration, expression of genes be regulated in macroglias, Nestin expression increase, and macroglia cells dedifferentiate after optic nerve injury. Autograft peripheral nerve can weaken the hibitory environment caused by optic nerve injury and promote the optic nerve regeneration. Autograft peripheral nerves can regulate gene expressions in macroglia cells and induce the dedifferentiation of macroglia cells in the optic nerve.更多还原