【作者】 杨红美；

【导师】 曾建国； 陈波；

【作者基本信息】 湖南师范大学 ， 分析化学， 2006， 硕士

【摘要】 中药活性成分的分离纯化和分析是中药现代化的重要组成部分。长久以来,中药质量控制缺少明确的指标、相关的标准对照品和有效的方法,对中药产品走向国际市场影响很大。本学位论文就目前中药质量控制存在的问题,采用现代色谱分离纯化和分析技术如:大孔吸附树脂、制备高效液相色谱和高效液相色谱-质谱联用等技术对几种中药中的活性成分进行分离纯化和分析方面的研究工作。 本论文主要的研究工作如下: 1.以制备木兰碱和衡州乌药碱的标准对照品为目标,应用大孔吸附树脂和制备高效液相色谱技术分离纯化美洲花椒中木兰碱和衡州乌药碱。考察了大孔吸附树脂和制备高效液相色谱的分离条件。该方法首次采用大孔吸附树脂进行制备产品脱盐处理且该方法具有生产周期短、产率高、产品纯度可以达到95%以上等优点。 2.建立同时测定虎杖及其提取物中白藜芦醇、大黄素、大黄酚、大黄素甲醚四种有效成分含量的高效液相色谱法。该方法可用于虎杖药材及其提取物的质量控制。 3.应用高效液相色谱、二极管阵列检测器和电喷雾质谱联用技术分析黄芩及黄芩提取物中黄酮类化合物,并以外标法对其中的8种成分定量分析,建立了一个较为全面的黄芩提取物的质量控制方法。 4.首次应用高效液相色谱、二极管阵列检测器和电喷雾质谱联用更多还原

【Abstract】 Separation and analysis of active components in traditional Chinese medicine is playing the vital role in the modernization of traditional Chinese herb medicine. Because of shortage of standards, analytical methods and quality control indexes, the products of traditional Chinese medicine have had difficulties to enter international market in a long time. The modern chromatographic separation and analysis technologies have been used to solve the problem that existed in quality control of traditional Chinese medicine. In this article, macro-porous adsorption resin chromatography technology, preparative high performance liquid chromatography technology, high performance liquid chromatography technology and high performance liquid chromatography-mass spectrometry technology have been used to separate and analyze active ingredients in several traditional Chinese medicines.The studies in this thesis are summarized as follows:1. In order to purify and separate magnoflorine and laurifoline in Zanthoxylum americanum, preparative HPLC and macro-porous adsorption resin chromatography technologies have been used to purify and separate magnoflorine and laurifoline. Conditions of separation preparative HPLC and macro-porous adsorption resin chromatography have been optimized. Macro-porous adsorption resin chromatography technology firstly used to remove the salt that existed in the product. Magnoflorine and laurifoline can been used as standards to control quality of the product.2. A HPLC method for simultaneous determination of resveratrol, emodin, chrysophanol, physcion in root of Polygonum cuspidatum and its extract has been established. The method is accurate, simple and reliable. It can be used for quality control of Polygonum cuspidatum and its extract.3. A novel method of simultaneous analysis and identification of eleven flavones in the medicinal plant of Scutellaria baicalensis using high-performance liquid chromatography (HPLC)-diode array detection (DAD)-electrospray mass spectrometry has been developed. Eight components could be quantified by using external standard method with UV detecting at 276nm. The samples analyzed contained roots, leaves, stems and extracts of Scutellaria baicalensis. The method can be used for quality control of extracts of Scutellaria baicalensis.4. A HPLC-ESI-MS method for simultaneous determination of flavones in seeds of the medicinal plant Oroxylum indicum has been established. Two components could be quantified by using external standard method with UV detecting at 276nm. The method is accurate, reliable. It can be used for quality control of Oroxylum indicum.更多还原