【作者】 易娇；

【导师】 尹笃林； 廉世勋；

【作者基本信息】 湖南师范大学 ， 有机化学， 2006， 硕士

【摘要】 烟草化学分析推动了烟草工业的健康发展。高效液相色谱法作为一种广泛的分离和分析技术,对烟草中化学成分的分析具有非常重要的应用价值。本论文建立了用高效液相色谱同时快速测定烟草中六种有机酸的方法,以及有机酸和多酚的同时快速高效液相色谱测定方法。并将其应用于考察转光地膜和转光反光地膜对烟草生长和化学成分的影响。主要内容如下: 1、建立了用高效液相色谱法一次进样、同时快速测定烟叶中六种有机酸的方法,对检测波长、流动相、洗脱方式进行了优化。该方法具有较高的灵敏度和精密度以及较好的重现性。并且分析时间不超过7min,同时对样品的前处理进行了优化。 2、建立了烟叶中有机酸和多酚的高效液相色谱同时快速梯度分析方法,分析时间不超过20min。对检测波长、流动相、洗脱方式以及样品的超声辅助提取条件进行了优化。并将该方法应用于分析烟叶中的四种主要有机酸和四种主要多酚化合物。 3、将建立的高效液相色谱法应用于考察转光地膜对烟叶中两种主要有机酸和多酚含量的影响,结果表明:转光地膜覆盖对烟株生长前期青烟叶中苹果酸的积累要大于普通对照膜CK,对柠檬酸的积累要小于普通对照膜CK;转光地膜覆盖下的绿原酸和芸香苷含量普遍下降,而普通对照膜CK均增加。烟草到达成熟期,转光地膜处理后普遍更有利于烟叶中绿原酸、芸香苷以及上部叶柠檬酸的积累,不利于苹果酸和靠近下部叶柠檬酸的积累。另外,还考察了转光地膜对烟草生长的影响,结果表明:转光地膜覆盖更有利于烟株根、茎、叶的生长发育;与普通对照膜CK相比,转光地膜具有更好的增温效果,并且有利于净光合率的增加以及叶绿素a、叶绿素b和总叶绿素含量更多还原

【Abstract】 Tobacco chemical analysis plays a very important role in the healthy development of tobacco industry. High performance liquid chromatography (HPLC) known as a kind of separating and analysis technology has a high practice value to analysis of chemical composition in tobacco. In this thesis, a method for simultaneous and rapid determination of 6 organic acids in tobacco leaf by high performance liquid chromatography was developed, and established a new HPLC method for the rapid simultaneous determination of organic acids and polyphenols in tobacco leaf. Then applying it to study the effect of sunlight-conversion film (SCF) and sunlight-conversion and reflection film (SCRF) on growth of tobacco and compounds. The main results are summarized as follows:1. A method for simultaneous and rapid determination of 6 organic acids in tobacco leaf by reverse-phase HPLC was developed, and 6 organic acids were identified and quantified in less than 7 min. Optimal conditions for separation of 6 organic acids, such as wavelength, mobile phase, elute mode were investigated. The method appears the higher sensitivity, precision and better repeatability. At the same time, preparation of the sample was optimized.2. A reversed-phase (HPLC) method was developed for the rapid simultaneous determination of organic acids and polyphenols in tobacco leaf, and they were identified and quantified in less than 20 min. Optimal conditions for separation of the main 4 organic acids and 4 polyphenols, such as wavelength, mobile phase, elute mode were investigated. Furthermore, the conditions of ultrasonic extraction were optimized.Followed by this method to analysis the main 4 organic acids and 4 polyphenols in tobacco leaf.3. The effect of SCF on the main 2 organic acids and 2 polyphenols in tobacco leaf was studied by using the fore mentioned HPLC method. The results indicated that in the growth prophase of tobacco, SCF had more effect on the accumulation of malic acid than CK, but for citric acid, it was contrary. Chlorogenic acid and rutin contents commonly decreased for SCF but increased for CK. In tobacco aging period, SCF had more effect on the accumulation of Chlorogenic acid, rutin in tobacco leaf and citric acid in above leaf than CK, but for malic acid and citric acid in below leaf, it was contrary.At the same time, the effect of SCF on tobacco growth was showed as follows: SCF was more propitious to grow for root, stem and leaf. Comparing to CK, it improved higher ground temperature. Furthermore, it increased the net photosynthesis velocity and improved the contents of chlorophyll a, chlorophyll b and total chlorophyll.4. The effect of SCRF on the main 2 organic acids and 2 polyphenols in tobacco leaf was studied by using the fore mentioned HPLC method. Through a series of researches, it was found as follows: In tobacco aging period, SCRF was commonly propitious to accumulate malic acid, citric acid, chlorogenic acid and rutin in below leaf, but it was more effect for malic acid and citric acid. In addition, we studied the effect of SCRF on thickness of foot leaf. It was found that the thickness of flue-cure tobacco leaf (underside three leaf location) of SCRF was thicker than CK, and then the foot leaf was more sufficient to be utilized.更多还原