【作者】 曹蕾；

【导师】 黄超文； 郑澜；

【作者基本信息】 湖南师范大学 ， 运动人体科学， 2006， 硕士

【摘要】 许多研究认为高原训练能够提高运动员的运动成绩是通过改变血浆Epo的含量,Epo与其受体EpoR结合后发挥生物效力,使外周血中红细胞数和血红蛋白含量升高,从而提高运动成绩,但近年来对血浆中Epo的研究并未取得一致结果。因此,我们试图以高住低练的训练方式来研究在不同低氧时间和递增运动强度下EpoR蛋白表达和EpoR mRNA的变化,进一步明确EpoR在“高住低练”中的变化机制。 研究方法:健康雄性2.0月龄Sprague-Dawley大鼠56只,随机分为7组:训练0天组(n=8)、高住低练3天组(n=8)、低住低练3天组(n=8)、高住低练6天组(n=8)、低住低练6天组(n=8)、高住低练13天组(n=8)、低住低练13天组(n=8)。采用递增运动强度,跑台坡度为0。大鼠运动后取双侧股骨骨髓。采用免疫组织化学、计算机显微图像分析、反转录多聚酶链反应(real time-polymerase chain reaction,RT-PCR)法进行实验,检测EpoR在骨髓组织中的蛋白和基因的水平,分析运动和低氧对骨髓EpoR蛋白表达以及EpoR mRNA的效应。 结果:(1)“高住低练”和“低住低练”都可使EpoR蛋白表达增加。高住低练中随着的训练时间的延长,EpoR增加更明显。 (2)“高住高练”组大鼠骨髓细胞EpoR mRNA表达显著上调(p<0.05),随着训练时间的延长大鼠骨髓细胞EpoR mRNA表达量逐渐增加,但无显著性差异(p>0.05);“低住低练”组大鼠骨髓细胞EpoR mRNA表达较训练前有所升高,但无显著性差异(p>0.05)。 结论:随着低氧和训练因素的影响,EpoR蛋白和mRNA均有逐渐上升的趋势,“高住低练”能使EpoR蛋白表达加快并上调EpoR的mRNA。更多还原

【Abstract】 It is well known that training high can improve the achievements of athletes. By changing the content of Epo in plasma, after Epo combining its receptor which is called EpoR, they can do biologic effect and raise the amount of RBC and Hb in the blood, improve the performance of exercise. But the research of Epo in plasma hasn’t reached the same result in recent years, so we intend to find the orderliness of express of EpoR protein to identify the relationship of living high training low and EpoR. Under different hypoxia time and increase by degrees of intensity exercise, we studied the changing of express of EpoR protein by living high and training low style.Methods: 56 healthy male SD rats were randomly divided into 7 groups: Living low no training (n=8), living high training low for 3 days(n=8), living low training low for 3 days(n=8), living high training low for 6 days(n=8), living low training low for 6 days(n=8), living high training low for 13 days(n=8), living low training low for 13 days(n=8). Under the condition of degrees of intensity exercises and gradient of zero. Take the arterial blood in the left ventricle of the male SD rats and their marrow in both the sides femur. Then, immunohistochemistry and computer image processing method and inverse transcription-polymerase chain reaction(RT-PCR) were used to examine the content of the EpoR protein express and mRNA in the marrow tissue and analyze the domino effect of the exercises and hypoxia on the EpoR albumen express .Results:(l)EpoR of bone marrow were increased both living high training low group and living low training low group. In the former group, the time of training were longer ,the increase of erythropoietin were more obviously.(2)EpoR mRNA of bone marrow cell were increased living high training low group obviously (p<0.05) . EpoR mRNA were increased depended on training time.Conclusion: Both EpoR protein and EpoR mRNA were increased in Living high training low .更多还原