【作者】 王兰；

【导师】 庄惠生；

【作者基本信息】 东华大学 ， 环境工程， 2006， 硕士

【摘要】 环境激素又称为内分泌干扰物,是一种由于人类的生产和生活活动而释放到周围环境中的,对人体内和动物体内原本营造的正常激素功能施加影响,从而影响内分泌系统的化学物质。它们能影响人类等多种生物的生殖功能与发育系统,降低生殖和免疫能力,导致神经系统失调及多种生理异常。近年来,环境激素问题已经成为继温室效应、臭氧层破坏之后的第三大环境问题,对其筛选及检测方法的研究,现已成为环境科学研究的热点领域之一。 本文研究了检测环境激素中双酚A（BPA）和2,4,6-三氯苯酚（TCP）的荧光免疫分析法及检测对叔丁基苯酚的流动注射化学发光法。这两种方法用于检测这三种环境激素物质均可达到比较理想的效果。 通过制备BPA和TCP的特异性抗体,建立荧光免疫分析法。先在半抗原BPA和TCP分子上引入羧基,然后将这两种半抗原与载体蛋白牛血清（BSA）偶联,合成全抗原。对合成的抗原进行紫外光谱扫描,确证偶联成功。在优化免疫学发应条件后,建立了测定BPA的抗原包被荧光免疫分析法和测定TCP的直接竞争荧光免疫分析法。 采用抗原包被荧光免疫分析法测定BPA,线性范围为10^-7～10^-4mg/mL,检出限IC₂₀为3×10^-7mg/mL。并将该法用于测定桶装饮用水和河水中的BPA,回收率在96%～108%之间。这种方法灵敏更多还原

【Abstract】 Environmental hormones, also called environmental endocrine disruption chemicals, act as estrogens in human and animal bodies. Most of them are discharged into the environment with the production and the life of the humanity. It has been found that environmental hormones existing in the environment have adverse effects on endocrine systems in human and animals and lead to reproductive, developmental and behavioral disorder. In recent years, environmental hormones have become the third environmental problem after the global warming and ozonosphere destruction. Now the research on the screening and determing the environmental hormones has become one of the hotspots of environmental science.Two new methods were researched in this paper: immunofluorescence method and flow injection chemiluminescence method, bisphenol A (BPA) 、 2,4,6-trichlorophenol(TCP) and p-tert-butylphenol were determined respectively. The determination of these three environmental hormones could achieve comparatively perfect results by using these two methods.Immunofluorescence method was developed for the analysis of BPA and TCP. First, the haptens of BPA and TCP were synthesized. Then thehaptens were conjugated to protein BSA to prepare antigens. The conjugates were identified by the method of ultraviolet. The result demonstrated that the conjugates were synthesized successfully. The antibodies were obtained after three months by injecting the immunogen in rabbits. Using the purified antiserum of highest specificity, an antigen coated immunofluorescence method and a direct competitive immunofluorescence method were developed.With the antigen coated immunofluorescence method, BPA was determined in the drinking water and river water. The linear calibration range was 10"7~10"4mg/mL, the detection limit IC20 was 3xlO~7mg/mL and the recoveries were between 96%~108%. The result was satisfactory.With the direct competitive immunofluorescence method, TCP was determined in the tap water and river water. The linear calibration range was 10’6~5xl0"4mg/mL, the detection limit IC2o was 2.4xlO~6mg/mL and the recoveries were between 95%~106%. The result was satisfactory.The chemiluminescence behavior of Luminol-H2O2 and p-tert-butylphenol in alkaline solution was researched by flow injection analysis. The related factors affecting chemiluminescence intensity were optimized. The method for determination of p-tert-butylphenol was established by flow-injection-chemiluminescence. The detection limit obtained was 4xlO"7 mg/mL. The linear range was 2*10’6~ lxlO’4 mg/mL. The relative standard deviation of 1.45% was obtained by 11parallel determinations of 10"5 mg/mL p-tert-butylphenol. This method was satisfactory for the determination of p-tert-butylphenol in water.Wang Lan (environmental science and engineering) Supervised by professor Zhuang Huisheng更多还原