【作者】 于学燕；

【导师】 王永刚；

【作者基本信息】 山东大学 ， 肿瘤学， 2005， 硕士

【摘要】 目的:研究基质金属蛋白酶(matrix metalloproteinases,MMPs)MMP-2和MMP-3在非小细胞肺癌组织中的表达,探讨两个指标与非小细胞肺癌患者的性别、年龄、肿瘤瘤体大小,病理类型、组织分化程度、临床分期、淋巴结转移等临床病理特征的关系。 方法:用免疫组化S-P染色技术对45例非小细胞肺癌标本及20例癌旁正常肺组织标本中MMP-2和MMP-3的表达水平进行检测,并结合患者的临床病理资料对其结果进行对比分析。 结果:MMP-2在非小细胞肺癌组织中的表达:(—)者12例、(+)者18例、(++)者15例、(+++)者0例,MMP-2在20例正常肺组织中的表达:(—)者16例、(+)者4例,(++)及(+++)者均为0例,统计学分析差异具有显著性(X~2=16.06,P<0.05)。 MMP-3在非小细胞肺癌组织中的表达:(-)者3例、(+)者24例、(++)者6例、(+++)者0例,MMP-3在20例正常肺组织中均无表达,统计学分析差异具有显著性(X~2=52.75,P<0.05)。 MMP-2、MMP-3在非小细胞肺癌中的阳性表达率与肺癌病理分型,患者性别、年龄、肿瘤瘤体的大小等因素无明显关系(P>0.05)。MMP-2的阳性表达率在非小细胞肺癌组织高分化与低分化者,有淋巴结转移和无淋巴结转移者,及不同临床分期的表达经统计学分析有相关性,而MMP-3的阳性表达与以上三种临床病理特征无相关性。更多还原

【Abstract】 Objectives: To investigate the expression of MMP-2 and MMP-3 in human non-small cell lung cancer and to evaluate how the two indicators relate to patients’ sex, age, tumor size, pathological type, and clinic phrase, etc.Methods: The expression of MMP-2 and MMP-3 was detected in 45 NSCLC samples and 20 para-cancerous normal lung tissues by immunohisto- chemical method, and the results were compared with consideration to patient’s clinicopathological data.Results: The expression of MMP-2 in NSCLC werel2 negative, 18 + cases, 15 ++ cases, zero +++ cases, the expression of MMP-2 in normal lung tissues were 16 negative, 4 + cases, and no ++ and +++ cases. The difference is statistically significant (X~2 =16.06, P<0.05).The expression of MMP-3 in NSCLC were 3 negative, 24 + cases, 6 ++ cases, no +++ cases. MMP-3 was not expressed in all 20 normal lung tissues. The difference is statistically significant (X~2 =52.75, P<0.05).Positive expression rates of MMP-2 and MMP-3 in NSCLC have no significant link with pathological type, age, sex, tumor size (P>0.05). The positive expression of MMP-2 was associated with TNM stage, pathological differentiation and lymph node metastasis (P<0.05). However, the expression of MMP-3 in NSCLC tissues was notassociated with patients’ clinicopathological features (P>0.05).Conclusion Expression of MMP-2 in NSCLC is different within patients who have different TNM stage, pathological differentiation and lymph node metastasis: Expression of MMP-2 in tissue of high differentiation is lower than that of low differentiation;with lymph node metastasis is higher than without metastasis;TNM III-IV is higher than I-II.Expression of MMP-3 in NSCLC does not show significant difference between different differentiation grades, with or without lymph node metastasis, and different TNM phrases.MMP-2 and MMP-3 were good tumor markers. The expression of MMP-2 is closely correlated with the invasion and metastasis of lung carcinoma. The expression of MMP-3 may be not a critical factor that affects the invasion and metastasis of lung carcinoma.Significance: research on the expression of MMP-2 and MMP-3 may improve the understanding of biological features at generic level on invasion, spread, and metastasis, facilitate the clinical diagnosis on metastasis and relapse, seek new treatment target, and meliorate patients’ prognosis.更多还原