【作者】 贺江萍；

【导师】 段宏泉；

【作者基本信息】 天津大学 ， 药物分析， 2005， 硕士

【摘要】 昆明山海棠(Tripterygium hypoglaucum level. Hutch)为卫矛科(Celastraceae)雷公藤属(Tripterygium)植物,是一种民间传统中药,具有消炎、解毒、祛风湿等作用。临床用于类风湿性关节炎、慢性肾炎和各种皮肤病。由于昆明山海棠具有良好的药理作用,国内外学者对其生药学、化学成分、药理作用、临床应用等方面进行了较多的研究和报道。该中药片剂收载于1995年版、2000年版、2005年版《中华人民共和国药典》。为进一步研究其有效成分,完善其质量控制,对其进行了相关的化学研究。以抗炎免疫抑制活性筛选结合各种提取分离技术,从昆明山海棠药材中分离得到两种二萜类化合物,并运用各种有机波谱的数据,鉴定了两个二萜类化合物分别为triptoquinone B和H;并将triptoquinone B和H进行了淋巴细胞转化实验,证实了其具有较强的免疫抑制活性。文献检索表明triptoquinone B和H也是卫矛科雷公藤属药用植物雷公藤(T. wilfordii Hook f)和东北雷公藤(T. regelii Spraque et Tak)中的有效成分,本文针对现有雷公藤类制剂的质量控制现状,首次建立了二萜类有效成分triptoquinoneB和H的高效液相色谱含量测定方法,并以此方法检测了雷公藤属药材及其制剂中triptoquinone B和H的含量,指出了提高有毒中药材及其制剂质量控制水平的重要性和研究发展方向。更多还原

【Abstract】 Tripterygium hypoglaucum level. Hutch (Celastraceae) is a folk medicine fortreatment of inflammation and arthritis diseases from ancient times. In recent years,its preparation was used for the treament of rheumatoid arthritis, nephritis and otherautoimmune diseases in clinic. Many studies on pharmacognosy, phytochemistry,pharmacology and clinical treatment of T. hypoglaucum have been carried out. Itstablet preparation is recorded in Chinese Pharmacopeia of 1995’s edition, 2000’s and2005’s edition. Further study on bioactive constituents and quality control for itspreparation were undertaken.Two immunosuppressive compounds were isolated from T. hypoglaucum byrepeated column chromatography. Their structures were identified as triptoquinone Band H by spectroscopic method. Triptoquinone B and H revealed the significantimmunosuppressive activity in the lymphocyte transformation test. Furthermore, triptoquinone B and H were also the active compounds of T.wilfordii and T. regelii reported in literature. The content determination oftriptoquinone B and H were established by HPLC method, and the content oftriptoquinone B and H in the plant of Tripterygium genus and their preparations weredetermined. The discussion of analysis result showed the essentiality of the qualitycontrol for about the poisonous medicinal plants and their preparations.更多还原