【作者】 付聪；

【导师】 成国祥；

【作者基本信息】 天津大学 ， 材料学， 2004， 硕士

【摘要】 分子印迹技术是制备对特定分子具有专一识别性能的聚合物的技术,分子印迹聚合物对模板分子的识别具有预定性、专一性和实用性等优点而在生物模拟、分离提纯、免疫测定以及痕量分析等领域显示出广阔的应用前景。本文对分子印迹技术的基本原理、分子印迹聚合物的制备和应用进行了较为全面的总述,对分子印迹技术当前存在的问题和未来的发展趋势进行了分析和展望。分别采用乳液聚合法、种子溶胀悬浮聚合法和预分散悬浮聚合法,在水相中制备了西咪替丁和酪氨酸印迹的分子印迹聚合物微球; 采用扫描电镜、光学显微镜及粒径分布仪对制备所得的分子印迹聚合物微球进行了表征; 采用紫外分光光度计对产物的吸附性能和对印迹分子的识别性能进行了研究。研究表明,乳液聚合法能够制备得到0.16-0.56μm之间的分子印迹聚合物微球,乳化剂、加料方式以及聚合体系的pH值是影响产物粒子形态的主要因素。种子溶胀悬浮聚合法制备得到50-400μm的分子印迹聚合物微球,种球性能和分散剂对微球粒径的影响比较大。预分散悬浮聚合法制备得到30-50μm分子印迹聚合物微球,产物粒径分布窄,制备工艺比较简单省时。本文所得的分子印迹聚合物微球对印迹分子的吸附满足Langmiur吸附模型,其吸附性能受到粒径、模板分子相对用量和致孔剂的影响比较大。所有分子印迹聚合物微球对模板分子都表现出优异的识别特性。乳液聚合产物分离因子α高达1.70,种子溶胀悬浮聚合物产物α在1.50左右,预分散悬浮聚合产物α基本保持在1.60。更多还原

【Abstract】 Molecular imprinting technique is a new method of preparing polymers that contains tailor-made recognition sides for certain molecules. It has predeterminative, specific and practical ability of molecule recognition. It is applied in biomimics, purification, separation and enrichment fields extensively. This thesis summarizes the development of the preparation and application of molecularly imprinted polymers (MIPs) and offers an insight in the near future. Some Cimetidine and Tyrosine imprinted polymer microspheres were prepared via emulsion polymerization; seeds swelling suspension polymerization and predispersed suspension polymerization respectively. Scanning electron microscope, optic microscope and particle size analyzer were used to analyze the properties of the products. The adsorption properties and recognition of imprinted molecular of MIPS in water was studied through ultra-spectrometer. It was showed that molecular imprinted polymer microspheres (MIPMs) between 0.16-0.56μm were prepared via emulsion polymerization method. Emulsifier, mode of monomers-addition and pH value of the polymerizing system, mainly influenced the morphology of the MIPMs. MIPMs between 50-400μm could be prepared via seeds swelling suspension polymerization. The particle size was influenced by seeds and dispersant. MIPMs between 30-50μm with narrower size distribution were prepared via predispersed suspension polymerization which was easier and more time-saving then the former. MIPMs prepared in this thesis adsorbed their templates in the way of Langmiur. The adsorption properties were influenced by particle size; template molecular relative value and progen. The MIPMs all exhibited a specific recognition to their templates. The separating factor α of MIPMs, prepared via emulsion polymerization; seeds swelling suspension polymerization and predispersed suspension polymerization, were about 1.70, 1.50 and 1.60 respectively.更多还原