【作者】 吴斌；

【导师】 袁先厚；

【作者基本信息】 武汉大学 ， 外科学， 2005， 硕士

【摘要】 目的:探讨P57^KIP2蛋白、Cyclin E蛋白在人脑胶质瘤中的表达及其与肿瘤细胞增殖活性的关系。 方法:采用免疫组织化学S-P法检测P57^KIP2蛋白、Cyclin E蛋白、PCNA蛋白在46例脑胶质瘤组织中的表达。 结果:在46例人脑胶质瘤组织中,P57^KIP2蛋白16例表达阳性占34.8%,显著低于正常脑组织（p<0.05）并与脑胶质瘤组织分化程度（Ⅰ—Ⅱ,Ⅲ—Ⅳ级）（p<0.05）及两年生存时间有关（p<0.05）。P57^KIP2蛋白的表达与Cyclin E蛋白、PCNA蛋白的表达密切相关（分别为r_s=-0.359,r_s=-0.502）。Cyclin E蛋白阳性率为58.7%（27/46）,显著高于正常脑组织（P<0.05）,且也与脑胶质瘤组织分化程度（Ⅰ—Ⅱ,Ⅲ—Ⅳ级）及两年生存时间有关（P<0.05）。Cyclin E蛋白的表达与PCNA蛋白的表达密切相关（P<0.01,r_s=0.451）。 结论:P57^KIP2蛋白的表达下调和Cyclin E蛋白的过度表达在脑胶质瘤的发生及恶性进展中起重要作用。P57^KIP2蛋白、Cyclin E蛋白是胶质瘤细胞增殖活性的重要调控因子,它们共同参与肿瘤细胞增殖的调控。它们表达水平的改变反映了肿瘤细胞增殖活性的改变,导致肿瘤细胞的增殖失去抑制,引起肿瘤的发生及恶性进展。P57^KIP2、Cyclin E和PCNA的联合检测有利于更准确地判断胶质瘤细胞的增殖活性和预后。更多还原

【Abstract】 Objective: To explore the expression of P57^KIP2 protein Cyclin E protein in human gliomas and their relationship with proliferating activity of tumor cell.Methods: The expression of P57^KIP2 protein Cyclin E protein and PCNA protein in 46 glioma tissues was detected by SP immunohistochemical technique.Results: The P57^KIP2 protein positive-expression rate in human gliomas was 34.8%, which was lower than that in normal brain tissue （P < 0.05）. The P57^KIP2 protein positive-expression correlated significantly with the differentiation degree of human glioma （P < 0.05） and the two-year survival time （P < 0.05） . The Cyclin E positive-expression rate in human gliomas was 58.7%.The Cyclin E positive-expression also correlated significantly with the differentiation degree of human glioma （P < 0.05） and the two-year survival time （P < 0.05）. The expression of P57^KIP2 protein was significant correlation with the expression of Cyclin E protein（rs = -0.359, P < 0.05） and PCNA protein（rs = -0.502, P < 0.01）. The expression of Cyclin E protein was significant correlation with the expression of PCNA protein（rs =0.451, P < 0.01）.Conclusion: P57^KIP2 protein and Cyclin E protein plays an important role in the development and malignant progress of human glioma. The expression level of P57^KIP2 protein and CyclinE protein correlated significantly with proliferating activity of tumor cell.The vary of its expression level indicated the vary of the proliferating activity of human glioma.Combined detection of P57^KIP2 Cyclin E and PCNA protein is helpful to judge the proliferating activity of tumor cell and prognosis.更多还原