【作者】 刘雪莉；

【导师】 张彤；

【作者基本信息】 新疆医科大学 ， 免疫学， 2005， 硕士

【摘要】 目的:研究HLA-DR1、3、15等位基因与新疆地区汉族人群变应性鼻炎(Allergic rhinitis,AR)的关联及比较相应位点下IL-4、IFN-γ水平。方法:采用聚合酶链反应序列特异性引物(PCR-ssp)技术对42例新疆地区汉族变应性鼻炎患者(变应性鼻炎组,AR组)的HLA-DR1、3、15等位基因进行检测,另选择同地区45例体检健康者(对照组)进行对照研究。同时采用双抗夹心法检测AR组和对照组外周血血清IL-4、IFN-γ的水平。结果:变应性鼻炎(AR)组HLA-DR1、15等位基因频率高于对照组(p<0.05)。AR组IL-4平均水平、IL-4/IFN-γ比值高于正常对照组(p<0.01),IFN-γ平均水平低于正常对照组(p<0.01)。AR患者组内HLA-DR15等位基因阳性者IL-4平均水平高于HLA-DR15等位基因阴性者(p<0.05)。结论:HLA-DR1和HLA-DR15等位基因与新疆地区汉族人群变应性鼻炎有关联,而与HLA-DR3等位基因未发现有明确的关联性。HLA-DR15阳性患者IL-4(Th2类细胞因子)平均水平高于HLA-DR15阴性患者。更多还原

【Abstract】 Objective: To study the association between HLA-DR1 、 3、 15 genes and the feature of allergic rhinitis and to compare the level of IL-4 and IFN-y cytokine among a xinjiang population of the han nationality. Methods: HLA-DR1 、 3、 15 alleles were conducted in 42 patients with allergic rhinitis and 45 normal controls from xinjiang with Polymerase Chain Reaction-sequence specific primer(PCR-ssp). The level of IL-4 and IFN-y were detected by ELISA. Results: the frequency of HLA-DR1、 15 is higher in patients with allergic rhinitis(AR) than that in normal controls (p<0.05). the averrage level of IL-4 and the ratio of IL-4/IFN-γ is higher in patients with allergic rhinitis(AR) than that in normal controls (p<0.01).the averrage level of IFN-γ is lower than that in normal controls (p<0.01). the averrage level of IL-4 is higher in HLA-DR15 gene positives than that in HLA-DR15 gene negatives in patitents with AR (p<0.05). Conclusion: patients with AR is associated with high frequency of HLA-DR1 and HLA-DR 15, HLA-DR3 is not associated with AR.the level of IL-4 cytokine is higher in HLA-DR 15 gene positives than that in HLA-DR15 gene negatives in patients with AR.更多还原