【作者】 刘军；

【导师】 甘子明；

【作者基本信息】 新疆医科大学 ， 人体解剖与组织胚胎学， 2005， 硕士

【摘要】 目的:探讨乙醇诱导大鼠急性胃炎后Fos在中枢神经系统及NOS在胃粘膜的表达及其意义。方法:动物被随机分为5组,每组10只。A组:单纯生理盐水灌胃组;B组:单纯乙醇灌胃组;C组:膈下迷走神经切断乙醇灌胃组;D组:内脏大、小神经切断乙醇灌胃组;E组:膈下迷走神经和内脏大、小神经均切断乙醇灌胃组。评定胃粘膜损伤指数(LI)及运用免疫组化技术显示Fos在大鼠中枢神经系统(CNS)中的分布和胃粘膜中NOS的变化。结果:B、C组动物的Fos阳性神经元在丘脑腹后外侧核,第9胸髓后角胶状质内大量存在,疑核和孤束核内有少量聚集;B、D组在疑核和孤束核内有较多聚集,但远低于B、C组在丘脑腹后外侧核、第9胸髓后角胶状质内的Fos阳性神经元数(P<0.05)。而A、E组在上述4个核团内Fos阳性神经元则较少。B、D组LI高于A组(P<0.05),而远低于C、E组(P<0.05)。在C、E组胃粘膜层及粘膜下层中只见稀疏的NOSⅠ阳性细胞,着色浅,而在A、B、D组胃粘膜层及粘膜下层见较密集的NOSⅠ阳性细胞,着色较深。在C、E组胃粘膜层及粘膜下层中只见稀疏的阳性细胞,着色浅。而NOSⅡ阳性细胞在C、E与B、D组胃粘膜层及粘膜下层中的分布正好与NOSⅠ阳性细胞的分布相反。结论:胃的痛觉冲动主要由交感神经传导,而迷走神经介导了胃感觉神经对胃体粘膜的保护作用。更多还原

【Abstract】 Objective: To study the expression and significance of Fos in central nervous system and NOS in gastric mucosa after ethanol-induced acute gastritis in rat. Methods: Animals were randomly classified into 5 groups:gaster-poured by physiological solution(A); gaster-poured by ethanol(B); gaster-poured by ethanol after hypophrenic vagotomy(C); gaster-poured by ethanol after big-small splanchnicotomy(D); gaster-poured by ethanol after hypophrenic vagotomy and big-small splanchni-cotomy(E). LI was studied.The distribution of Fos-positive neuron in central nervous system and NOS-positive cell in gastric mucosa was displayed and immunohistochemical staining method was used. Results: There were a lot of Fos-positive neuron in thalamus ventral-lateral nucleus and No 9 thoracic medullaris posterior horn cinerea in groups B and C; Fos-positive neuron in ambiguous nucleus and nucleus of solitary tract were better in groups B and D, but they were obiviously less than them of halamus ventral-lateral nucleus and No 9 thoracic medullaris posterior horncinerea in groups B and C(P<0.05). LI of groups B and D were higher than them of group A(P<0.05). but them were obiviously much higher than them of groups C and E(P<0.05). There were a lot of NOS I -positive cell in mucosa of gastric body in groups A、 B and D. And them were obiviously much more than them of groups C and E(P<0.05)There were a lot of NOS II -positive cell in mucosa of gastric body in groups C and E. And them were obiviously much more than them of groups A、 B and D(P<0.05). Conclusion: The gastric algesia impulsion are mainly conducted by sympathetic afferent nerve. And NO protecting mucosa of gastric body are mainly introduced by vagus nerve.更多还原