【作者】 牛艳；

【导师】 许兴； 徐兆桢；

【作者基本信息】 宁夏大学 ， 作物栽培学与耕作学， 2005， 硕士

【摘要】 本研究以宁夏枸杞主栽品种“宁杞1号”为材料,采用高效液相色谱分析方法（HPLC）对在宁夏不同枸杞产区（中宁、银川、同心、惠农）的宁夏枸杞中重要有效成分（β-胡萝卜素、甜菜碱、牛磺酸等）进行了测定比较。同时,对其土壤因子、气象因子进行了测定分析,最后研究了生态因子对枸杞果实中的有效成分积累的影响。结果表明: 1.高效液相色谱方法测定宁夏不同地区的宁夏枸杞中主要成分（β-胡萝卜素、甜菜碱、牛磺酸等）含量,方法简单,分析时间短,准确度高。高效液相色谱（HPLC）图谱信息量较大,对有标准品的中药及制剂的质量提供较全面、可靠的科学评价,达到中药识别和半定量,定量及各成分相对比较定量,对中药及制剂的质量控制十分有效。 2.宁夏枸杞中各个有效成分的含量在各地区分别有所不同:表现为惠农枸杞中多糖含量最高达到了7.07%,中宁枸杞中β-胡萝卜素含量为最高,达到了127.12μg,中宁枸杞中甜菜碱含量为最高,达到了2.298mg,园林场宁夏枸杞中牛磺酸含量最高达到了4.069mg,不同地域宁夏枸杞中黄酮含量之间存在极显著差异,表现为宁夏枸杞栽种老产区中宁的枸杞中黄酮含量为最高,达到了5.575mg,相反,惠农的枸杞中黄酮含量最低,只有1.316mg,占中宁枸杞的23.61%。从总体水平上分析看,宁夏中宁县和惠农县产的枸杞中所含的有效成分都较高,均达到了优质枸杞的水平。 3.宁夏四个主要产区土壤和枸杞中所含的微量元素含量差异不显著,但从微量元素的协同效应来看,中宁枸杞较高的Fe/Mn值,使的其质量优手其他三区。虽然四个产区土壤Fe元素含量无显著差异,但从四区土壤PH看,中宁土壤PH为7.4,而其它三区土壤PH在8.5以上,土壤偏碱性,使Fe元素活性钝化,降低植物对其有效吸收,因而使惠农、同心、园林场三产区的枸杞Fe/Mn值低于中宁的。 4.分别对宁夏四区枸杞主要有效成分含量与生态因子关系分析发现,惠农、同心两地土壤盐分较高,其果实内的多糖含量也较高,说明一定浓度的NaCl对多糖含量有一定的促进作用,土壤全盐含量是影响枸杞多糖含量的第一因子。土壤全盐与枸杞甜菜碱含量有显著的正相关性,表明一定浓度的盐分对甜菜碱的积累有一定的促进作用。宁夏枸杞牛磺酸含量与盐分没有关系,与主要盐分组成离子也没有相关性,盐分对牛磺酸的合成积累没有影响。一定浓度的CaSO₄和CaCl₂对黄酮含量的积累有一定的抑制作用,初步认为盐分制约黄酮含量的更多还原

【Abstract】 Several physiological characters of Ningqi No.1 of lycium barbarum L of Ningxia breed are compared under different conditions. Room experiments are carried out in order to compare the main compositions contents of Lycium barbarum L.in different regions through developing HPLC(high press liquid chromatogram). At the same time ,detennination and analysis on the soil factors and weather condition, together with the concentration of main effective compositions of lycium barbarum L. are undertook at four production in Ningxia, namely Tongxin,Huinong ,Zhongning and Yinchuain. The results are shown as follow:1. (8-carotenids, Taurine, Betain) contents of Lycium barbarum L.in Ningxia different regions were tested by the means of HPLC, whose method is simply, shortly, accurately. Information content in HPLC-finger print is rich, which provides comprehensive, reliable scientifically appraise to some Chinese medicine and preparation with standards, and realizes the identification, half-quantification, quantification, relative quantification of every contents. It is every effect to quantities control of Chinese medicine and preparation.2. The soil salinity of four different areas which the Ningqi No 1 have been planted in Ningxia are analyzed. The results demonstrated that the salt in the soil of Tongxin and Huinong are comparatively higher and therefore ,the concentrations of Polysaccharomyces are also higher if compared with other two districts .One of the main construction ions,Na~+and Cl~- have significant relativity with Polysaccharomyces in the seeds of lycium barbarum.L. The correlation between polysaccharomyces and the general salt in the seeds of lycium barbarum L is both positive and significant. The result proves that a defined concentration of NaCl improves the accumulation of polysaccharomyces in the seeds of lycium barbarum L on some extent.3. Through the relativity analysis of the important valid components of lycium barbarum L. and soil ecosystem factor in four areas of Ningxia, sifting the environment factors which affect on the valid components of lycium barbarum L. On this foundation, building up the multiple equations of linear regression .The results showthat the contents of valid components were affected together by soil factors and weather condition, .but soil factors affected contents more greatly. However, it does not know that ecosystem factor of soil how to affect on it’s the accumulating mechanism, still need the further research.4. The trace elements have not significance difference in the soil and fruit of Tongxin, Zhongning, Huinong, Yanlinchang. From the point of trance element, quality of the fruit of Lycium barbarun L of several main producing regions in Ningxia didn’t have a remarkable difference each other, but considered from cooperative effect of trace element, four of Ningxia produce regions, the ratio of Fe/Mn in Zhongning was higher than other areas and had significance difference, the soil PH in Zhongning is 7.4, but others are higher than 8.5, which makes active of Fe blunt, it is difficult to absorb for plant. So proper trace element ratio was a main reason in being of the Lycium barbarum L of Zhongning in best quality than other regions.5. Find out through analytical detection, the contents of the valid component of Lycium barbarum L. have the dissimilarity in everyplace in four main regions of Ningxia. Seeing from the total level up, the contents valid components of Lycium barbarum L are higher in Zhongning and Huinong, all come to a high-quality, express that these two regions are a most beneficial region for lycium barbarum L to grow, its soil and weather conditions are suitable the growth of the lycium barbarum L very much, can produce a fruit of good quality, is one of the index signs of the lycium barbarum L of inborn now.更多还原