【作者】 关宇光；

【导师】 王廷华；

【作者基本信息】 昆明医学院 ， 人体解剖与组织胚胎学， 2005， 硕士

【摘要】 目的:探讨Fas、FasL在成年灵长类动物恒河猴中枢神经系统(Central Nervous System, CNS)的分布及脊髓损伤后的表达变化。 方法:取成年健康恒河猴18只,随机分为6组,每组3只。其中5组在脊髓的T11部位半横断制作脊髓半横断模型,模型动物分别手术后7d、15d、30d、60d、90d不同部位取材。采用CNS组织切片免疫组织化学SP法染色,显微镜下观察Fas、FasL免疫阳性反应物在CNS的分布,计数各组损伤部位整个脊髓前角、后角及大脑皮质运动区的Fas、FasL阳性神经元数,结果用多因素析因方差分析、LSD检验进行统计学处理。 结果:Fas、FasL的阳性表达在正常对照组恒河猴CNS的分布不同,小脑皮质梨状细胞层的Purkinge细胞胞浆和突起呈Fas阳性反应,而FasL阳性神经元分布于小脑神经核;间脑中有FasL阳性神经元分布;在脑干的神经核团内Fas和FasL都有分布;在脊髓,Fas、FasL阳性神经元以前角大运动神经元最为明显,染色较深,白质内的胶质细胞和神经纤维亦有阳性反应,二种因子的阳性反应均定位于胞浆和突起。脊髓半横断后各时间段大脑皮层运动区及脊髓后角未见明显Fas、FasL阳性神经细胞分布,其免疫阳性产物主要定位于脊髓灰质前角神经元胞浆和突起。脊髓半横断后其损伤部位上下端前角Fas、FasL阳性神经元数的总体变化趋势是7d组阳性神经元数最多,以后逐渐下降,60d后恢复到正常对照组水平。其中7d,15d半横断侧头端与尾端前角Fas、FasL阳性神经元数与对侧及正常组比较细胞数增加(P<0.05),半横断侧头端与尾端之间比较无统计学差异(P>0.05),半横断对侧脊髓前角Fas阳性神经元与正常组比较亦无显著性差异(P>0.05)。其它时间段各组与正常组比较及组间比较无统计学差异(P>0.05)。 结论:Fas、FasL在CNS的多个部位都有表达,但各有侧重,提示这二种因子在维持CNS的免疫及生理功能方面可能发挥作用。脊髓半横断损伤后脊髓前角神经元Fas与FasL表达共同经历了一个由增高到逐渐降低的过程。Fas与FasL的表达变化趋势相似,在脊髓更多还原

【Abstract】 Objective: To investigate the distribution of Fas and FasL in the Central Nervous System (CNS) of adult rhesus and the changes of expression of Fas and FasL in the hemisected spinal cord of adult rhesus.Methods: 18 adult male rhesus were used. They were divided into six groups randomly. Spinal cord hemisection were performed at T11 level. The anaminals were allowed to survive for 7 days, 15 days, 30 days, 60 days, and 90 days. The CNS in control group were obtained in order to investigate the change and distribution of Fas and FasL as well as the spinal cord and moter region of certex in experimental groups. The data were statistically analysed with factorial ANOVA and LSD test.Results: The Fas and FasL immunopositive neurons were observed in many areas. Fas immunoreactivity can be seen in the cytoplasm and processes of Purkinge cells and brain stem nuclei. FasL immunopositive neurons were observed in cerebellar nuclei, diencephalon and brain stem nuclei. Fas and FasL immunoreactivity mainly distributed in motor neurons of spinal ventral horn and neural fibers and glia cells in white matter. Brown staining was observed in the cytoplasm and process.Fas and FasL immunoreactive products deposit in the cytoplasm and processes of stained neurons distributed in the ventral horn of all rhesus and the number reached maximumly 7 days post operation (dpo), then declined and returned to normal up to 2 months post operation in all operated groups.The total number of immunoreactive neurons in the caudal and rostral spinal cord of the rhesus 7dpo and 15dpo on the operative side had significant increase than that ofnormal control group (P<0.05) and that in the contralateral side (P<0.05). There were no statistic difference between the caudal and rostral spinal cord of all rhesus on the operative side (P>0.05). In addition, Fas and FasL had the same expressional trend after the injury.Conclusion: Fas and FasL distributed in various areas of CNS, which indicated that they might play some roles in the immune and physical function of the aforesaid sites.In the ventral horn of hemisection rhesus Fas and FasL had the same expressional trend, that is reached maximumly 7 dpo, then declined and returned to normal up to30 dpo or 60 dpo post operation, which showed that Fas and FasL might involve in the apoptosis and secondary injury in the spinal cord and provide the theory basement for the therapy of spinal cord injury in clinic.更多还原