【作者】 周治；

【导师】 解保生； 徐芸；

【作者基本信息】 昆明医学院 ， 口腔临床医学， 2005， 硕士

【摘要】 错(牙合)畸形的矫治已有百年历史,但是对于矫治过程当中出现的一系列变化和反应的机制仍不完全清楚。目前认为有多种生物因子在多个层次共同参与正畸过程中牙周组织改建。 神经生长因子(Nerve growth factor NGF)是一种靶源性神经营养因子,对神经系统及非神经系统有广泛的效应。已证明NGF在痛觉感受及软硬组织损伤修复方面有重要作用。正畸牙加力的初期会出现疼痛,随着力的持续,压力侧牙槽骨吸收,张力侧牙槽骨形成,牙齿发生移动。鉴于NGF在疼痛的发生及硬组织改建当中所起的重要作用,我们假设NGF在正畸力作用下牙周组织反应过程中发挥某种作用。三叉神经是颜面部主要感觉神经,有关牙髓损伤后三叉神经节内NGF表达变化已有报道,但正畸过程中三叉神经节内NGF表达变化,国内外均未见报道。 本研究选用105只雄性成年SD大鼠,随机分为空白组,加力对照组与加力组,各组又分为1、3、5、7、9、14、21天组,每组各5只。模拟临床牙受力过程,采用免疫组织化学方法检测三叉神经节内NGF的表达。探讨NGF与正畸期间机体反应之间的关系,为深入了解正畸牙移动机制提供参考。 结果: 大鼠牙齿受力后,第1天即观察到三叉神经节内NGF表达明显增强,至第3天达到最高,以后逐渐下降,但仍高于两对照组。 本研究结果表明大鼠牙受力初期,NGF表达显著增高,可能与矫治初期疼痛有关,以后逐渐下降,但仍高于对照组,提示NGF可能在后期牙周组织改建更多还原

【Abstract】 The treatment of malocclusion has been more than one hundred years, but the mechanism of a serial of changes and reactions in orthodontics has not been understood exactly. It has been thought that there are many factors involved in different stages of teeth movement.Nerve growth factor (NGF) is one of neurotrophins. It affects both on nervous and non-nervous system. It has been well known that NGF affects occurrence of inflammation pain and organism restoration. Generally, pain would occur during the beginning of force applied. Alveolar bone of the compressed area would be absorbed with the last of force and that of the tension area formed, then teeth moved. In view of the role of NGF may involve pain and organism restoration, we assume NGF might play an important role during orthodontic teeth movement. Trigeminal ganglia are main sensory nerve of face. It has been reported that the expression changes of NGF in trigeminal ganglia after teeth injured. However, the expression changes of NGF in trigeminal ganglia during orthodontic teeth movement keep to being unknown.105 adult male SD rats were divided into control group, sham experimental group and experimental group. Above groups were divided into 1, 3, 5, 7, 9, 14, 21 days sub-groups. Force was applied to first maxillary molars of right side which analyzed teeth movement. The expression changes of NGF in trigeminal ganglia were investigated by immunohistochemistry, in order to know the intercourse of NGF and orthodontic teeth movement.Results:After force applied on teeth, NGF expression increased greatly and heightened to maximum in the group of 3 days. From the groups of 5 to 21 days, the NGFexpression descended gradually, but was higher than that of control groups.This investigation showed NGF expression increased greatly at the beginning of force applied, which may be related to orthodontic pain. NGF expression descended gradually later, but was higher than that of control groups, which suggested NGF might be involved in regeneration of alveolar bone.更多还原