【作者】 陈强；

【导师】 林芳灿；

【作者基本信息】 华中农业大学 ， 微生物学， 2005， 硕士

【摘要】 以香菇的4个栽培菌株和6个野生菌株为材料,利用原生质体单核化技术获得单核体,取每一菌株的1个单核体作杂交亲本,进行所有可能组合即C₁₀²个组合的杂交,从中得到36个杂交双核菌株。 将10个亲本菌株和36个杂交菌株分别接种在以淀粉和葡萄糖为主要碳源、并添加不同浓度的2—脱氧葡萄糖（2DG）的培养基上,比较它们的菌落生长速率、菌丝平均直径、菌丝体生物量以及以葡萄糖为主要碳源的培养基中葡萄糖的消耗。并考查不同浓度2—脱氧葡萄糖对以上各项性状的影响,检测各菌株对2—脱氧葡萄糖的抗性。 结果发现,在46个供试菌株中,在以淀粉为碳源时,2DG抗性和强抗性菌株各有11株和19株;在以葡萄糖为碳源时,10个亲本对2DG表现抗性,36个杂交菌株对2DG表现强抗性。 为了探讨产量与2DG抗性之间的关系,进行了含4次重复的随机区组试验。结果表明:结实率高,出菇早,产量高的菌株大多数是在以淀粉为主要碳源时对2—脱氧葡萄糖具抗性的菌株。更多还原

【Abstract】 Monokaryons of 4 cultivated strains and 6 wild strains of Lendnula edodes were obtained via protoplast monokaryotization technology. Crosses were made among 10 monokaryons which had been chosen one from the ten dikaryotic strains in allpossible combinations , C₁₀², and 36 dikaryotic strains were gained by mono-mono mating among these ten monokaryotic strains.All dikaryotic strains were inoculated on the media using starch and glucose as main carbon sources respectively, and different concentrate 2-deoxy-glucose （2DG） was added. It was carried out to compare their colony growth rate, average diameter of hypha, mycelia biomass and glucose consumption on the media using glucose as main carbon source. Their resistances to 2-deoxy-glucose were measured, and the effects of different concentrate 2DG to those characters above identified.The result shows that among 46 strains used in this study, eleven are resistant and nineteen are strong resistant to 2DG using starch as main source; while all parental strains are resistant to 2DG and all hybrid strains are strong resistant to 2DG using glucose as main source.A randomized block test with 4 replication was made for approaching the relation between production and 2DG resistance. Comprehensively analyzing the results, the conclusion was that most strains which form fruiting bodies easily, fruit early and yield highly are resistant to 2DG on the media in which starch is main carbon source.更多还原