秦艽地上部分化学成分的研究熊胆粉中牛磺熊去氧胆酸钠和牛磺鹅去氧胆酸钠的分离与含量测定

【作者】 赵勇；

【导师】 孙文基；

【作者基本信息】 西北大学 ， 中药学， 2005， 硕士

【摘要】 1.秦艽为龙胆科植物大叶秦艽（Gentiana macropylla Pall.）、麻花秦艽（Gentiana straminea Maxim.）、粗茎秦艽（Gentiana crassicaulis Duthie ex Burk.）和小秦艽（Gentiana dahurica Fisch.）的干燥根,是我国重要的传统中药之一,其主要有效成分为龙胆苦苷,具有抗炎、镇痛、保肝、利胆等多种药理作用具有广泛的开发及应用前景。本文从充分利用资源和扩大入药部位出发,对秦艽地上部分化学成分进行了一定研究,运用多种分离方法分离得到6种化合物,并运用波谱学手段(UV、IR、MS、¹³C-NMR、¹H-NMR、HMBC、HMQC、H-H COSY),结合理化常数、标准对照品,鉴定了6种化合物的结构。同时采用正交试验法优选出从秦艽地上部分提取龙胆总苷的最佳提取工艺;通过不同型号大孔吸附树脂对龙胆苦苷吸附效果的比较,确定了用于富集龙胆苦苷的大孔吸附树脂的型号;并通过其对龙胆苦苷的吸附和洗脱试验,确定了最佳工艺条件。此外,采用HPLC法对秦艽地上部分不同部位龙胆苦苷进行了定量分析;为陕西省教育厅重点科研项目“秦艽地上部分综合开发利用”进行了基础研究。 2.熊胆粉为熊科动物棕熊Vrsus arctor L. 或黑熊Selenarctor thibetanrs Cuvier经胆囊手术引流胆汁而得的干燥品。临床作为熊胆药物使用,属国家卫生部批准的一类新药,其主要化学成分为结合型胆酸。本文采用经典柱层析法和高效制备液相色谱法相结合从熊胆粉中分离得到牛磺熊去氧胆酸钠和牛磺鹅去氧胆酸钠两种差向异构体,并采用ELSD—HPLC法对熊胆粉中牛磺熊去氧胆酸钠和牛磺鹅去氧胆酸钠进行了定量分析。更多还原

【Abstract】 1 Qinjiao is the roots of Gentiana macrophylla Pall.、 Gentiana straminea Maxim.、Gentiana crassicaulis Duthie ex Burk and Gentiana dahurica Fisch. Which belongs to Genus Gentiana. It is an important traditional Chinese drug . The main component gentiopicroside have the effects of anti - inflammatory, stopping pains, protecting liver and Lidan. And its prospects is broad. In order to make full use of the resource and expand the using parts of Qinjiao ,we carried out a systematic research on the chemical constituents in the aerial parts of it .Six compounds were achieved by using several separation methods. The structures of them were authenticated by the means of spectroscopy (UV、IR、MS、~13C-NMR、~1H-NMR、 HMBC、HMQC etc.) The optimum extract method was selected by using of orthogonal experiment ,and the optimum model of resin was selected by comparing the adsorptional ability of different resins to gentiopicroside. Meanwhile by doing adsorption and unadsorption experiments, the best technological conditions was determined. Besides the content of gentiopicroside in different aerial parts was determined by HPLC. We have done some basic researches for the important project of Shannxi educational department -"the comprehensive development and utilization of the aerial part of Qinjiao".2 bear biliary drainage powder was the dry bile of Vrsus arctor L or Selenarctor thibetanrs Cuvier. It is used as bear bile in clinic, and belongs to the first category new medicine which was ratified by the Ministry of Health. The main components were combine cholic acid. We get the sodium tauroursodeoxycholate and sodium taurocheno- deoxycholate from bear biliary drainage powder by using of the classical separation method and high performance preparative liquid chromatography. Meanwhile we determined the content of sodium tauroursodeoxycholate and sodium taurochenodeoxy- cholate in bear biliary drainage powder by ELSD-HPLC .更多还原