人B淋巴细胞刺激因子载体稳定性及其嵌合毒素的表达载体构建与产物性质鉴定

【作者】 吴玉荣；

【导师】 张双全；

【作者基本信息】 南京师范大学 ， 生物化学与分子生物学， 2005， 硕士

【摘要】 人B淋巴细胞刺激因子(human B Lymphocyte Stimulator,hBlyS)是一种B淋巴细胞的共刺激因子,属TNF超家族成员。BLyS有三个受体BAFF-R,BCMA和TACI,三个受体主要表达于B淋巴细胞。BLyS在Anti-IgM或IL-4存在下能专一的刺激B细胞增殖、分化及分泌免疫球蛋白,在体液免疫中有重要的作用。BLyS同自身免疫性疾病有关,在很多的淋巴瘤的发生中有作用。 嵌合毒素是具有导向能力的分子(载体)和具有细胞毒性的分子(毒素)偶联而成的具有特异性细胞杀伤能力的杂合分子。载体主要使用抗体及细胞因子,毒素主要使用超抗原及蛋白质合成抑制剂等。嵌合毒素在肿瘤的治疗上有其突出的优点:对肿瘤细胞具有选择性杀伤作用,细胞毒效价高,无抗药性。 本论文详细介绍:本室构建的重组pET30a/hsBLyS质粒在BL21(DE3)菌株中的稳定性观察,嵌合毒素hsBLyS-PE40的基因构建、表达及活性检测。更多还原

【Abstract】 Human B Lymphocyte Stimulator(hBLyS) is a new member of tumor necrosis factor(TNF) superfamily. BLyS has three receptors BAFF-R、 BCMA and TACI, all of them are expressed in B cells. It stimulates proliferation of, and immunoglobulin production by, B cells in the presence of Anti-IgM or Interleukin-4(IL-4), BLyS plays an important role in the regulation of humoral immunity. BLyS also plays a role in autoimmune diseases. Clinical studies suggest a potential role for BLyS in the progress of lymphoid malignancies.Chimeric toxin is a heterogeneous molecule that is composed of homing molecule and cytotoxic molecule. Homing molecules are commonly antibodies and cytokines, toxins are almost super-antibodies and inhibitors of protein synthesis. Chimeric toxin has many advantages in tumor therapy: selective killing tumor cells, high cytotoxity effect, no drug fastness.In this paper we describe in detail study on the heredity stability of a recombinant plasmid pET30a/hsBLyS in Ecoli BL21 and construction 、 expression and activity detection of the chimeric toxin hsBLyS-PE40.更多还原