【作者】 周祥；

【导师】 张肇达；

【作者基本信息】 四川大学 ， 外科学， 2005， 硕士

【摘要】 目的:探讨胆汁中K-ras基因突变在胰腺癌肝转移的早期诊断中的价值。方法:通过建立胰腺癌及胰腺癌肝转移的大鼠动物模型,收集模型大鼠的胆汁,采用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)检查胆汁中K-ras基因12密码子点突变情况并与病理切片法作对比性分析。结果:16例胰腺癌动物模型胆汁标本中无K-ras基因突变(0/16),19例胰腺癌肝转移动物模型胆汁标本中K-ras基因突变率为84.2%(16/19)。胰腺癌肝转移胆汁K-ras基因突变检测的敏感性为66.7%,特异性为81%,阳性预测值为80%,阴性预测值为85%。结论:胰腺癌肝转移组动物模型胆汁中K-ras基因可以发生突变,其与胰腺癌肝转移相关性好,突变率高,特异性强,可能为胰腺癌肝转移早期诊断的初步筛选提供新的方法。更多还原

【Abstract】 Objective: To investigate the value of K-ras mutation in bile for the earlydiagnosis of hepatic metastasis in pancreatic cancer.Methods: Buliding pancreatic cancer and hepatic metastasis in pancreaticcancer of animal model with rats ,we collected bile from them ,and used thepolymerase chain reaction and restriction fragment-length polymorphism(PCR-RFLP) to evaluate K-ras point mutation at code 12 in bile. Then, theresult was compared with pathologyic slice up..Results: The positivity of K-ras point mutation in bile of hepatic metastasis inpancreatic cancer was 84.2%(16/19), and that was 0.0%(0/16) in model withpancreatic cancer.The sensitivity of surveying K-ras mutation in bile of hepatic metastasis inpancreatic cancer was 66.7%, the specificity was 81.0%, the positive predictionwas 80.0%, and the negative prediction was 85.0%.Conclusions: It suggests that K-ras point mutation at codon 12 in bile is athigh risk for development of hepatic metastasis in pancreatic cancer. Both themutability and the specificity of K-ras are high .Our work can supply a newelemental filter method for the early diagnosis of hepatic metastasis in pancreatic cancer.更多还原