【作者】 徐新运；

【导师】 周雪；

【作者基本信息】 四川大学 ， 人体解剖学与组织胚胎学， 2005， 硕士

【摘要】 目的:探讨凋亡相关因子Bax、Bcl-2及Caspase-3在正常猫L₆背根节、部分去背根猫的针刺侧及非针刺侧备用背根节的表达情况,以了解部分去背根及针刺对三因子在备用背根节表达的影响。 方法:成年健康雄猫20只随机分为4组:即正常组、备用根术后单侧针刺3天组、7天组及14天组（动物行双侧部分去背根手术,即切除两侧L₁-L₅、L₇-S₂背根节,保留双侧L₆为备用根,针刺备用背根节外周支配区内的两组穴位:足三里和悬钟,伏兔和三阴交。针刺频率98次/分,时间为30分钟,每15分钟时交换电极,每天针刺1次,每次针刺一组穴位,两组穴位交替进行。）。动物分别于术后3天、7天及14天处死。取正常组左右侧、手术组两侧的L₆背根节,-20℃恒冷箱切片,片厚20μm,分别用兔抗Bax（1:500）、兔抗Bcl-2（1:500）及兔抗Caspase-3（1:500）抗体行免疫组化ABC法染色。观察、计数并比较各组L₆背根节中Bax、Bcl-2及Caspase-3的分布及阳性神经元数。进行组间多个样本均数比较的单因素方差分析、q检验和组内两样本均数比较的t检验。 结果:更多还原

【Abstract】 Objective: To explore the expression of Bax, Bcl-2 and Caspase-3 in spared dorsal root ganglion after partial dorsal root rhizotomy and acupuncture of adult cats.Methods: 20 adult cats were divided into 4 groups: normal control group; bilateral partial dorsal root rhizotomy and acupuncture 3^rd, 7^th and 14^th day groups （bilateral L1-L5, L7-S2 DRGs were transected, but L₆ DRG was spared, electro-needle stimulation was performed following bilateral partial root rhizotomy at the right side） . Animals survived for 3, 7 and 14 days after operation respectively. Bilateral L₆ dorsal root ganglia were made into frozen sections（20μm）. Then sections were stained under the same condition using specific Bax （1:500）, Bcl-2 （1:500） and Caspase-3（1:500） antibody by the immunohistochemistry ABC method. The distribution and the number of Bax. Bcl-2 and Caspase-3 positive neurons in bilateral spared DRG（L₆） were observed and counted. All data were analyzed30 by One-Way ANOVA. SNK-q test and paired-t test.Results: 1. The expression of Bax after partial dorsal rhizotomy and acupuncture in spared L₆ DRGIn normal adult cats, Bax immunoreactants were observed in partial large and medium-small sized neurons of L6 DRG The number of Bax positive total and medium-small sized neurons in spared DRG of the 3 day group increased apparently as compared with that of the normal control group （PO.05） but decreased apparently in the 14 day group and showed no significant difference when compared versus control （P >0.05 ） . Three days after acupuncture, the number of Bax positive total and medium-small sized neurons in acupuncture side had no significant change as compared with that of unacupuncture sidefT* >0.05). In 7 day group, Bax expression in neurons of DRG in acupuncture side decreased apparently as compared with that of unacupuncture side and returned to reference level and continued to 14 day.2. The expression of Bcl-2 after partial dorsal rhizotomy and acupuncture in spared I^DRGBcl-2 immunoreactants were observed in partial large and medium-small sized neurons of L& DRG. Bcl-2 expression among three operation groups in spared DRG had no difference compared with that of reference group （,P>0.05）. Three days after acupuncture, the number of Bcl-2 positive total and medium-small sized neurons in acupuncture side had no significant change compared with that of unacupuncture side and reference group. In 7 day group, the number of Bcl-2 positive total and medium-small sized neurons in acupuncture side increased compared with that of unacupuncture side and continued to 14 day group.3. The expression of Caspase-3 after partial dorsal rhizotomy and acupuncture in spared L6 DRGCaspase-3 immunoreactivity was observed in partial large andmedium-small sized neurons of L6 DRG. The number of Caspase-3 positive total and medium-small sized neurons in spared DRG of all three operation groups increased apparently as compared with that of reference group （P<0.05） . The number of Caspase-3 positive total and medium-small sized neurons in acupuncture side of all three operation groups decreased significantly as compared with that of unacupuncrure side. The number of Caspase-3 positive total and medium-small sized neurons in acupuncture side of both 3 day and 7 day groups had no significant difference compared with reference group（f>0.05） and that of 14 day group decreased apparently as compared with reference group（P<0.05）.4. The influence of partial dorsal rhizotomy and acupuncture on the expression of BaxN BcI-2^ Caspase-3 of different subpopulation neurons in spared DRGPartial dorsal rhizotomy and acupuncture on the expression of Bax^ Bc1-2n Caspase-3 of medium-small sized neurons in spared DRG had been mentioned, but had no significant influence on that of large neurons. Conclusion: Partial dorsal root rhizotomy mainly had an effect on the expression of Bax, Bcl-2 and Caspase-3 in medium-small sized neurons of spared DRG, especially from 3 day to 7day after the operation. The expression of Bax> Bcl-2 and Caspase-3 in spared DRG neurons were affected differently after acupuncture. Acupuncture mainly inhibited the expression of Bax and Caspase-3 but accelerated Bcl-2 expression in themedium-small neurons of spared DRG.更多还原