【作者】 叶纯；

【导师】 王凡；

【作者基本信息】 四川大学 ， 人体解剖与组织胚胎学， 2004， 硕士

【摘要】 目的:研究淫羊藿防治去势大鼠骨质疏松症的疗效及TNF-α、TGF-β₁在椎骨中的表达,探讨淫羊藿防治绝经后骨质疏松症的机理。 方法:采用3月龄SD雌性大鼠45只,随机分为正常对照组（1组）、去势组（2组）和实验组（3组）。2组及3组均切除双侧卵巢,3组给予淫羊藿水提液灌胃处理;1组给予假手术处理。分别于手术后4周、8周、12周,随机处死三组大鼠中各5只,取第五腰椎,脱钙后包埋切片。Van Gieson染色,图像分析骨小梁结构;HE染色,计数多核巨噬细胞/破骨样细胞;常规SABC法进行TNF-α、TGF-β₁免疫组化染色,图像分析灰度值。 结果:去势组与正常对照组比较,4周时出现骨小梁厚度下降,12周时骨小梁面积百分比下降明显,骨小梁分离度增加。实验组的骨小梁计量指标、多核巨噬细胞/破骨样细胞计数与对照组相似,与去势组比较具有显著差异。去势组骨髓单核细胞胞质中强阳性表达TNF-α,实验组及对照组呈弱阳性/阴性表达。TGF-β₁在实验组及对照组的成骨细胞中呈染色阳性,部分骨基质中也有阳性表达,三个时间点的去势组均呈阴性表达。 结论:本实验采用双侧卵巢切除的方法,12周后成功地制备了绝经后骨质疏松大鼠模型。淫羊藿阻止了椎骨早期骨丢失的发生,维持了骨小梁的形态,有效地预防了骨质疏松症的发生。淫羊藿能够持续地下调去势大鼠骨髓组织中单核细胞表达TNF-α,同时诱导骨微环境中TGF-β₁表达,进而抑制破骨样细胞的生成及活性。这可能与淫羊藿黄酮有着类雌激素的化学结构,并能通过与雌激素受体结合发挥拟雌激素样作用有关。更多还原

【Abstract】 Objective To study the therapeutic results and mechanism of Epimedium on preventing ovariectomy-induced osteoporosis and expression of TNF - α, TGF -β₁ in microenvironment of vertebra in rats.Methods 45 female SD rats at 3 months were randomly divided into control group （group 1）, tendency group （group 2） and experiment group （group 3）. Groups 2 and 3 all been amputated ovary, group 3 were given Epimedium extract. Group 1 were given to use skill handling. Termination of 5 rats in all groups took place respectively after operation 4 weeks, 8 weeks and 12 weeks. The 5th lumbar vertebra was taken and paraffin section was made. The samples were dyed with HE, Van Gieson and immunohistochemical stain of TNF - α and TGF-β₁. Bone little beam structure were analysed with Image Analyst, and multi-nuclei / osteoclast like-cells were counted.Results Compare with control group, Tb.Th and Tr.Ar have obvious decreasing while Tb.Sp increasing in tendency group. Trabecular bone in Epimedium group measure is similar to compare group. The number of multi-nuclei / osteoclast like-cells in tendency group is obviously more thangroup 1 and group 3. Expression of TNF-a showed that positive cells of monocytes in group 2 and weak positive or negative cells in group 1 and group 3. Osteoblasts and matrix of group 1 and 3 showed positive reaction of TGF-Pi but negative in group 2.Conclusion In this study, we successfully made a model of postmenopausal osteoporosis by ovariectomy. Epimedium can inhibit bone loss early and prevent efficiently the ovariectomy-induced osteoporosis. Epimedium obviously inhibit osteoclasteogenesis and bone resorption by down-regulate the expression of TNF-a, and induce the expression of TGF-Pi in microenvironment. We suppose that flavonoids, which the main component of Epimedium, can result in this effect because it4ias resemble chemistry structure as estrogen.更多还原