【作者】 余兰；

【导师】 魏世成；

【作者基本信息】 四川大学 ， 口腔临床医学， 2004， 硕士

【摘要】 对腭裂、外伤、肿瘤等造成的腭部软硬组织缺损,临床上常规采用软组织瓣转移修复,存在一定的不足。本实验针对常规手术方法存在的缺陷,探索新的修复方法,将生物相容性良好的纳米羟基磷灰石/聚酰胺复合人工骨n-HA/PA66制成板状,植入羊腭部人工造裂区,探讨n-HA/PA66人工骨板修复重建腭部软硬组织缺损的可能性。 纳米羟基磷灰石/聚酰胺复合人工骨材料制备成厚为1.00mm的板状。实验动物选用山羊8只,随机分为实验和空白对照两组,每组4只。于羊硬腭中份做15.0×20.0mm~2的矩形洞穿性缺损。实验组植入纳米人工骨板。空白对照组做同样大小的软硬组织缺损,但不植入人工骨板。术后通过大体、X摄片、组织学和电镜观察,评价其对腭部软硬组织缺损修复重建情况和新生组织结构。 结果如下:实验组组织沿人工骨表面逐渐爬行生长,6周内完全关闭软组织;16周关闭硬组织缺损,人工骨与软硬组织结合紧密;空白对照组软硬组织不能自行愈合。新生组织结构与正常组织结构类似,组织内未见明显排异反应。 综上所述,n-HA/PA66人工骨板具备组织支架桥梁和GBR的双重作用,可引导羊腭部组织沿其生长,自行修复腭部软硬组织缺损。该材料及方法具有重要的临床开发运用价值。更多还原

【Abstract】 This study is to evaluate the use of nano-hydroxyapatite/pol yamide-66(n- HA/PA66) plate to reconstruct the bone and soft palatal defects caused by cleft palate , trauma and tumor in clinic,and to explore the possibility of repairing the tissue defects in the palate with n- HA/PA66.8 goats as experimental animals were divided into experimental group and control group at random. 15.0×20.0mm~2 rectangle tissue were removed in mid part of hard palates, n- HA/PA66 plate were implanted into the experimental group defects.The same size tissues in the control group were removed without the n- HA/PA66 plate implanted. In the 4,8,12,16 weeks after surgery,radiation, histological analysis and scanning electron microscope (SEM)observation were performed to evaluate whether the materials have effects on the reconstruction of the defects in the palates.The results are as the following ,the wound healed over after2weeks. There were significant differences between the experimental and control defect in two groups.In experimental group,the soft tissue regenerated along the n- HA/PA66 plate and defects were closed in 6weeks. The bone tissue regenerated along the n- HA/PA66 plate and defects were closed in 16weeks.The tissue did not close up in control group. No obvious rejection reaction could be observed.It is conclusioned that The experiments demonstrated that the n-HA/PA66 ’s biocompatibility and bioactivity are good. N- HA/PA66 can reconstruct the palate defect,and have the clinical application value.更多还原