【作者】 钟琳；

【导师】 熊英；

【作者基本信息】 四川大学 ， 儿科学， 2004， 硕士

【摘要】 目的 通过对地塞米松处理后缺氧缺血性脑损伤大鼠脑组织葡萄糖转运蛋白1在不同时点表达的检测,了解地塞米松对缺氧缺血性脑损伤大鼠脑内葡萄糖代谢的影响,探讨不同剂量地塞米松对葡萄糖代谢的作用。 材料与方法 90只7日龄SD大鼠,分为5组,每组18只,(1)对照组;(2)缺氧缺血组,腹腔注射生理盐水;(3)缺氧缺血后小剂量地塞米松给药组0.1mg/kg-d,腹腔内注射3天;(4)缺氧缺血后中剂量地塞米松给药组0.5mg/kg.d,腹腔内注射3天;(5)缺氧缺血后大剂量地塞米松给药组1mg/kg-d,腹腔内注射3天。制成缺氧缺血性脑损伤动物模型后,于即刻、6小时、12小时、24小时、72小时及7天处死,灌注后脑组织冰冻切片做免疫组化,观察GLUT1蛋白水平的变化。采用Image ProPlus4.5图像分析系统对图像进行光密度分析,用SPSS10.0进行统计分析,P<0.05有统计学差异。 结果 GLUT1在大鼠缺氧缺血性脑损伤后的表达比对照组明显增高(P更多还原

【Abstract】 ObjectiveWe designed this experiment to investigate the influence of dexamethasone (DEX) on expression of glucose transporter 1(GLUT1) in hypoxic-ischemic rat brain, to research whether the expression will change with different dosages of DEX at different time spots.Methods And Materials7-day old SD rats were divided into 5 groups: (1)control group(C), in which the rats were given false operation;(2)hypoxic-ischemic group(HI), vehicle were given i.p. for 3 days; (3) low dosage treatment of Dexamethasone group(LD),0.1mg/kg i.p. for 3 days following hypoxia-ischemia;(4)moderate dosage treatment of Dexamethasone group, 0.5mg/kg i.p. for 3 days following hypoxia-ischemia; (5) high dosage treatment of Dexamethasone group, 1mg/kg i.p. for 3 days following hypoxia-ischemia. The pups were perfused at specific intervals of recovery from hypoxia-ischemia. Immunohistochemical analysis of GLUT1 was adopted.ResultsThe GLUT1 signals in HI group are significantly higher than that in Control group(P<0.05), and different in recovery intervals. The signals in LD groups are higher than HI group but not significant(p>0.05). The GLUT1 signals in MD group are significantly higher than HI group’s (p< 0.05). The signals in HD group are higher at early stage(p<0.05) but drop to a lower level than HI group(p<0.05).ConclusionThe GLUT1 protein level significantly increased after hypoxic-ischemic insult of neonatal rat brain . And the Dexamethasone treatment promoted the expression of glucose transporter 1 protein after the hypoxic-ischemic insult of neonatal rat brain at early stage. A proper dosage of Dexamethasone treatment at early stage may protect the brain.更多还原