【作者】 刘勇；

【导师】 刘季春；

【作者基本信息】 江西医学院 ， 外科学， 2005， 硕士

【摘要】 目的: 研究食管鳞状细胞癌组织中基质金属蛋白酶-1(matrix metallopr-oteinase-1,MMP-1)及基质金属蛋白酶抑制剂-1(tissue inhibitor of metalloproteinases-1,TIMP-1)的表达与食管鳞状细胞癌生物学行为之间的关系,探讨二者与食管鳞状细胞癌组织分化程度、浸润深度、淋巴结转移的关系。方法: 收集2000 年3 月～2001 年11 月我院36 例食管鳞状细胞癌手术标本,均经病理诊断证实(年龄范围35 岁-70 岁;男29 例,女7例)。所有患者术前均未经化疗和放疗。其中高分化6 例,中分化15例,低分化15 例,有淋巴结转移14 例,无淋巴结转移22 例。癌组织穿透食管外膜25 例,未穿透食管外膜11 例。采用ElivisionTM plus 免疫组组织化学法检测食管鳞癌组织中MMP-1、TIMP-1 的表达。结果: 1、MMP-1 阳性染色主要见于肿瘤细胞,间质细胞也呈弱阳性表达。MMP-1 在食管鳞癌组织的阳性率为38.8%,MMP-1 表达与肿瘤分化程度无明显关系(P>0.05)。浸及外膜的食管癌组织中的阳性表达率明显高于未浸及外膜的食管癌组织中的阳性表达率(P<0.05);伴淋巴结转移组的阳性率较无转移组明显高(P<0.05)。更多还原

【Abstract】 Objective: To investigate expression of matrix metalloproteinase-1(MMP-1) and tissue inhibitor of metalloproteinases-1 (TIMP-1) in esophageal squmous carcinoma and the expression and the biological behavior of esophageal squmous carcinoma. Methods: Esophageal tumour samples(n=36) were obtained from esophagectomy specimens from patients(age range 35-70 years;29 males and 7 females) who had undergone surgery for esophageal cancer and none had receiced chemotherapy or radiotherapy.All specimens which had been submitted to the Department of Pathology in The First Affiliated Hospital of Jiang Xi Medical College(time range March 2000-November 2001).There are all squmous carcinoma.Among the 36 cases,6 tumors were well-differentiated squamous cell carcimomas,15 were moderately differentiated and 15 were poorly differdntiated;14 cases with lymph node metastases,22 cases with lymph node nometastases;11 cases invased to submucosal carcinomas and muscularis propria,25 cases invased to esophageal adventitia.The expression of MMP-1 and TIMP-1 of 36 cases of esophageal squmous carcinoma were detected by immunohistiochemistry(the method of ElivisionTM plus). Results: 1 、Immunoreactivity of MMP-1 is found evenly in the tumor cells,while rarely in the interstitial cell.The expressive rate of MMP-1 of esophageal squmous carcinoma was 38.8%,and the expressive rate of MMP-1 was independent of tumor differentiation(P>0.05),higher rate of lymph node metastasis(P<0.05),higher rate of cases invased to esophageal adventitia(P<0.05). 2、Immunoreactivity of TIMP-1 is found evenly in the tumor cells and interstitial cell. The expressive rate of TIMP-1 of esophageal squmous carcinoma was 50.2%, and the expressive rate of TIMP-1 was independent of tumor differentiation(P>0.05),lower rate of lymph node metastasis(P<0.05),lower rate of cases invased to esophageal adventitia(P<0.05). 3、The cases which the expression of MMP-1 preponderated over the expression of TIMP-1 easilier occurred lymph node metastasis than the cases which the expression of TIMP-1 preponderated over the expression of MMP-1. Conclusions: 1、The expression of MMP-1 and TIMP-1 has a relationship with the biological behavior of esophageal squmous carcinoma.The positive control for MMP-1,while the negative control for TIMP-1. 2 、The Blance MMP-TIMP which keep integrality and internal environment of extracellular matrix was determinant,and provided important target spot for the therapy of tumor.更多还原