【作者】 陈萍；

【导师】 丛雅琴； 侯明；

【作者基本信息】 山东大学 ， 内科学， 2005， 硕士

【摘要】 目的:研究垂体瘤转化基因(pituitary tumor-transformaing gene,PTTG)和c-myc基因在急性白血病(acute leukemia,AL)患者中的表达,探讨PTTG和c-myc基因与急性白血病的发生、化疗效果和预后因素之间的关系,为进一步研究PTTG在急性白血病发生中的作用机制提供理论基础。 方法:采用RT-PCR方法检测46例初诊急性白血病患者、14例未完全缓解(non-complete remission,NCR)患者、15例完全缓解(complete remission,CR)患者及10例正常对照骨髓单个核细胞(bone marrow mononuclear cell,BMMNC)中PTTG和c-myc基因的表达。 结果: 1.初诊急性白血病患者PTTG和c-myc基因的表达水平明显高于正常对照组(P<0.05),PTTG基因与c-myc基因的表达水平呈正相关(r=0.564,P<0.05)。 2.AML和ALL患者PTTG和c-myc基因的表达水平差异无统计学意义(P>0.05),ALL组PTTG表达水平高于AML组,但差别无统计学意义(P>0.05)。AML组c-myc基因的表达水平高于ALL组,差异无统计学意义(P>0.05)。 3.治疗后达CR患者组PTTG和c-myc基因的表达水平低于NCR组(P<0.05)。 4.CR组化疗前PTTG和c-myc基因的表达水平低于NCR组,差异有统计学意义(P<0.05)。 5.有异常染色体核型组PTTG和c-myc基因的表达水平高于无异常染色体核更多还原

【Abstract】 Objective: To explore the the expression of PTTG and c-myc gene in acute leukemia (AL) and its relationship with acute leukemia. To explore the correlation between overexpression of PTTG and abnormal expression of oncogene c-myc in AL, and chemotherapy effects and prognostic factors of AL.Methods: Expression of PTTG and c-myc gene mRNA were detected in BMMNC from 46 patients with untreated acute leukemia , 15 patients who reached CR , 14 patients who failed and 10 normal controls ,by reverse transcriptase-polymerase chain reation (RT-PCR) .Results:1. Expression of PTTG and c-myc gene in BMMNC were significantly higher in untreated AL patients than in normal controls( P<0.05) .2. The expression of PTTG and c-myc gene in AML and ALL showed no significance ( P>0.05). The expression of PTTG in ALL was higher than in AML but there showed no significance ( P>0.05). The expression of c-myc in AML was higher than in ALL but there showed no significance (P>0.05).3. Expression of PTTG and c-myc gene were lower in patients who reached CR than in patients who failed ( P< 0.05).4. Expression of PTTG and c-myc gene before chemotherapy were lower in patients who reached CR than in patients who failed ( P<0.05).5. Expression of PTTG and c-myc gene were higher in untreated patients withabnormal karyotype than in untreated patients without abnormal karyotype ( P <0.05). Conclusion:1. PTTG and c-myc mRNA were overexpressed in patients with AL.2. Overexpression of PTTG and c-myc mRNA were related to abnormalkaryotype.3. The effects of chemotherapy in the AL patients with overexpression of PTTGand c-myc gene were poor.4. PTTG may be a prognostic factor in acute leukemia patients.5. Overexpression of PTTG and c-myc mRNA in AL patients may be relatedto the progression and pathogenesis of acute leukemia.更多还原