【作者】 闫曰莲；

【导师】 陈建；

【作者基本信息】 山东大学 ， 内科学， 2005， 硕士

【摘要】 目的:探讨PCNA与Fas在胃癌组织中的表达及临床病理学特征和预后的关系。 方法:采用PV-9000通用型二步法免疫组化检测69例胃癌组织、24例肠上皮化生和22例不典型增生组织PCNA与Fas的表达,分析其与临床病理学特征的关系。统计学分析用X~2检验。 结果:PCNA在胃癌中的表达率(55.07%)显著高于其在肠上皮化生(25.00%)和不典型增生(27.27%)中的表达(P<0.05),且与病理组织学分级、肿瘤浸润深度、淋巴结转移情况、pTNM分期这些临床病理学参数有相关性(p<0.05),而与其他参数无关。Fas在胃癌中呈低表达(42.03%),显著低于其在肠上皮化生(83.33%)和不典型增生(81.82%)中的表达(p<0.01),且与病理组织学分级、pTNM分期有相关性(p<0.05),而与其他参数无关。另外,PCNA与Fas在胃癌中的表达呈负相关。 结论:PCNA与Fas分别反映了胃癌细胞增殖和凋亡的情况,PCNA在胃癌中的过表达与胃癌的进展关系密切,Fas在胃癌中低表达反映了其恶性程度的进展,是一个有价值的预后因子。PCNA与Fas在胃癌中的表达呈负相关,提示PCNA表达上调与Fas表达下调共同促进肿瘤进展。联合观察二者的变化可作为胃癌患者预后的重要指标。更多还原

【Abstract】 Objective: To study the protein expression of PCNA and Fas in the progression and prognosis of gastric carcinoma.Method: sixty-nine cases of gastric carcinoma (GC),twenty-four cases ofintestinal metaplasia (IM)and twenty-two cases of dysplasia (Dys) were studied by immunohistochemical technique .Statistical analysis of group differences was performed by x2 test.Results: The positive rate of PCNA in gastric carcinoma was 55.07%,significantly higher than that in IM(25.00%)and Dys(27.27%) (p<0. 05),and its expression was significantly related with tumor differentiation, infiltration ,lymph node metastasis and stage (p<0. 05) .The positive rate of Fas in gastric carcinoma was 42.03%, significantly lower than that in IM(83.33%) and Dys(81.82%) (p< 0. 01) ,and its expression was significantly related with tumor differention and stage(p <0.05) .Further more, the expression of PCNA was inversely correlated with the expression of Fas.Conclusion: the expression of PCNA and Fas could reflect the proliferative and apoptosis activity of gastric tumor cells respectively, PCNA overexpression may be related to progression in gastric carcinoma ,the reduction of Fas may be reflect the progression and high grade malignancy of GC and may be a useful indicator for prognosis. The overexpression of PCNA and reduction of Fas may play synergeticrole in the course and development of GC. Their expression may be helpful in determing rumor progression.更多还原