【作者】 郭莉；

【导师】 王正荣；

【作者基本信息】 四川大学 ， 生物医学工程， 2005， 硕士

【摘要】 目的 探讨不同光暗循环周期对小鼠活动性、外周血白细胞计数及分类计数、血清IgG浓度的影响,为进一步研究光对机体行为、免疫系统的影响提供理论依据;为时间生物学中,进一步揭示光与机体行为、免疫系统生物节律间的相互关系,打下一定的基础。 方法 将144只ICR小鼠随机置于三个不同的光暗循环周期LD7/7(7h光照/7h黑暗)、LD12/12(12h光照/12h黑暗)及LD16/16(16h光照/16h黑暗)下,喂养6周后,分批取血作白细胞计数及分类计数,采用双抗体夹心酶联免疫法测定血清IgG的浓度。另外9只ICR小鼠亦分为三组,红外线检测装置和数据分析系统连续6周监测其活动性的改变。 结果 (1)不同的光暗循环周期下,除嗜酸性和单核细胞外,小鼠活动性、白细胞计数及分类、血清IgG的大小不同。(2)小鼠活动性节律与三种光暗循环周期均同步。(3)小鼠白细胞计数及分类计数、血清IgG的节律与LD12/12的光暗循环同步;在LD7/7及LD16/16的条件下,则不存在节律性。更多还原

【Abstract】 Objective To investigate the effect of different light-dark cycles on locomotor activity , total and differential leukocyte count in peripheral blood as well as serum IgG concentration in mice. This study will give evidences for further researches on the light effect on organism behavior and immune system.Moreover, our researches will contribute to further studies on the relationship between light and biorhythms of the organism behavior and immune system in choronobiology.Methods 144 ICR mice were divided into three groups. Duringsix weeks the mice were exposed to one of three light-dark cycles regimes: LD7/7(7h light /7h dark),LD12/12(12h light /12h dark),LD16/16(16h light /16h dark). Blood samples were attained by enucleation of eye at 4 h intervals for total and differential leukocyte count as well as serum levels of IgG determined by enzyme-linked immuno-sorbent assay. Another nine mice were also divided intothree groups and their activities were recorded continuously and analyzed by computer.Results First, locomotor activity , total and differential leukocytecount as well as serum level of IgG in mice were distinct with different light-dark cycles except for acidophile and monocyte count .Second, locomotor activity rhythm in mice was synchronized to all three different light-dark cycles while total and differential leukocyte count as well as IgG rhythm wasn’t synchronized to the light -dark cycles except LD12/12 .Conclusions First, different light-dark cycles could influenceWBC count and differential blood count as well as serum IgG concentrations except for acidophile and monocyte count. Second, locomotor activity rhythm in mice can be entrained to all three different light-dark cycles while total and differential leukocyte count as well as IgG rhythm couldn’t be entrained to light-dark cycles correspondingly except LD12/12.更多还原