【作者】 陈家群；

【导师】 杨永亮；

【作者基本信息】 广西师范大学 ， 体育教育训练学， 2005， 硕士

【摘要】 力竭性运动时,随着机体内运动源性自由基的堆积,导致脂质过氧化反应加强,造成细胞膜结构和功能的改变,是产生运动性疲劳的重要原因。因此,及时、有效的清除过量的自由基,对延缓疲劳的发生有着重要意义。中药运动营养补剂具有经济性和毒副作用小等明显的优点。随着体育科研和竞技体育的迅速发展,在现代先进的实验条件下,中药运动营养补剂已进入了竞技体育的大门,并取得了显著的成果。但同时也存在不少的问题,由于所采用的研究方案、实验条件、测试指标、受试对象的来源、受试对象参加实验时的状态、疗程时间、力竭的判断、取样部位、取样时间、乃至检测仪器等都存在较大的差异,这些差异都可能影响中药抗疲劳药效的观察,很难对研究结果进行相互比较,更难于判断在被不同学者证明了具有明显功效的产品中,哪一种产品更好、更适合自己使用。因此,造模方法应予以标准化。从而便于对抗运动性疲劳的各种中药治法进行比较,探索、筛选最佳治法和方药。本文在查阅了青钱柳、罗汉果、Vc 的药用价值及临床应用的基础上,基于运动与自由基、青钱柳与自由基、罗汉果与自由基、Vc 与自由基之间的作用,比较研究了青钱柳、罗汉果、Vc 对运动能力、保肝、保心、清除自由基及抗运动性疲劳能力的影响。从而探索、筛选抗运动性疲劳效果更明显、作用更突出的产品,为青钱柳、罗汉果、Vc 作为运动保健食品、运动营养补剂的开发、利用提供理论及实验依据。第一部分研究工作,其目的在于探索不同剂量的青钱柳对小鼠运动能力的影响,为后续实验确定有效、科学的最佳灌胃剂量。本实验以8 周龄健康雄性昆明种小鼠为研究对象,随机分成6 组,每组10 只。分别为:空白对照组、剂量1组、剂量2 组、剂量3 组、剂量4 组、剂量5 组。实验中我们在毒性实验的基础上采用华罗庚的“黄金分割优选法”来确定各组的灌胃剂量,并以负重爬绳和负重游泳模型探索最佳灌胃剂量。实验结果显示:剂量3 组(20.84g/Kg·W·D)较对照组和其它剂量组对小鼠运动能力的提高具有更明显的作用。因此,确定剂更多还原

【Abstract】 In exhaustive exercise, with the accumulation of free radical in organics, the lipid peroxide reaction is strengthened, which result in the change of structure and function of cell membrane. And it is the important reason which produce fatigue after exercise. Therefore the effective disposal of free radicals in time bears important significance for the postponing of fatigue. Nutritional medicament made from TCM (traditional Chinese medicine) is economical and has little side effect. With the rapid development sports research and athletics, under the condition of modern advanced experiments, Nutritional medicament made from TCM has been used by the circle of athletics and sports and nutritional medicament made from TCM has produced marvelous effect. But at the same time, there exist many problems. Due to the marked difference in research scheme, experiment conditions, testing guidelines, the source of subject, period of treatment, the judgment of exhaustive exercise, the place of sample, the time of sampling and testing instrument which will affect the observation of TCM’s anti-fatigue effect, it is very difficult to compare and decide which product is best and more suitable for yourself. So the establishment of model should be standardized to facilitate the comparison, exploration and selection of the best treatment method among anti-fatigue TCM medicament. Based on the author’s investigation of the medical value and clinic application of Cyclocary Paliurus, momordica grosvenori and Vc as well as the interaction of mobile radical and free radical, Cyclocary Paliurus and free radical, momordica grosvenori and free radical, Vc and free radical, thesis compares and studies the effect of Cyclocary Paliurus momordica grosvenori and Vc on exercise competence, the protection of liver and heart and the disposal of free radical and anti-fatigue competence after exercise to explore and select anti-fatigue products with best effect and provide the theoretical and experimental proof for the development and utilization of Cyclocary Paliurus, momordica grosvenori and Vc as healthy food and nutritional medicament. The research in the first phrase aims at the effect of Cyclocary Paliurus on mouse’exercise competence and determine the efficient and scientific best amount of lavage. This experiment take healthy male Kunming mouse at the age of 8 as research object. And the mice are randomly divided into 6 groups, ten mice in a group. They are blank control group, dose group 1, dose group 2, dose group 3, dose group 4 and dose group 5. In this experiment, we determine the lavage dose based on toxic experiment, adopting Huang Luogeng’s “Golden Section and Optimization”and explore the best dose amount of lavage taking climbing rope with load and swimming with load as model. The result of the experiment show the effect of dose amount on the exercise competence is more obvious in dose group 3 (20.84g/kg.w.d) compared with other control groups or dose group. So the dose amount in dose group 3 is determined as the best dose amount for later experiments. The research in the second phrase aims at the comparison of the effect of momordica grosvenori VC on mouse’anti-fatigue competency to explore and select the products with obvious anti-fatigue effect. The thesis aims at providing theoretical and experimental foundation for the exploration and utilization of momordica grosvenori and Cyclocary Paliurus as health food and nutrition. This experiment takes healthy male Kunming mouse as research object. The mice are divided into 8 groups randomly and ten mice are in a group. They are: quiet control group, VC quiet group, Cyclocary Paliurus quiet group, momordica grosvenori quiet group, training control group, VC training group, Cyclocary Paliurus training group, momordica grosvenori training group. Mice in control group are lavaged with 0.4 ml salt water. Mice which take medicine are lavaged with certain amount of medicineaccording to the prescribed dose every day and this last 43 days. Mice in training group carry out six weeks of training with increasing load and before they do exhaustive exercise, mice are imposed with certain amount of load. And after they have a rest of 24 hours, a one-time model of exhaustive exercise is established. After exhaustive exercise, the blood is taken as sample to measure the vitality of SOD, GSH-Px in quadriceps femoris, the amount of MDA, the vitality of GPT, GOT, CK in blood, the amount of LD, BUN, Glu, Hb and the original amount of glycogen and inositol. The result of the experiment shows that: 1.to supply Cyclocary Paliurus momordica grosvenori and Vc doesn’t affect the weight of mouse. 2.to supply Cyclocary Paliurus with the dose of 20.84 g/kg.w.d can obviously improve the swimming competence of mice with load. 3.to supply Cyclocary Paliurus, momordica grosvenori and Vc can prolong the time of mouse with load swimming to exhaustiveness. It indicates that Cyclocary, momordica grosvenori and Vc have best anti-fatigue effect and the effect of Cyclocary is best than momordica grosvenori and Vc. 4.to supply Cyclocary Paliurus, momordica grosvenori and Vc can control the decrease of Hb in mouse, which is beneficial to the increase of metabolism and postpone the happening of fatigue after excercise. And the effect of Cyclocary is best than momordica grosvenori and Vc. 5.to supply Cyclocary Paliurus, momordica grosvenori and Vc can control the production of LD in exercise and accelerate the disposal of LD. And the effect of Cyclocary is best than momordica grosvenori and Vc. 6.to supply Cyclocary Paliurus, momordica grosvenori and Vc can obviously decrease the damnification of mice’s histiocyte of cardiac muscle, liver and boned muscle after exhaustive exercise. It can be shown by the fact that the vitality of blood serum GOP, GTP and CK of mice which take medicine and training after exhaustive exercise is lower than that of mice in training control group. 7.to supply Cyclocary Paliurus, momordica grosvenori and Vc is beneficial to thedecrease of decomposition of protein and amino acid. And the effect of Cyclocary is best than momordica grosvenori and Vc. 8.to supply Cyclocary Paliurus, momordica grosvenori and Vc can improve the reserves of hepatin to ensure providing energy for central nerves system, red blood cell and boned muscle. And it can improve exercise competence and postpone the happening of fatigue after exercise. And the effect of Cyclocary is best than momordica grosvenori and Vc. 9.to supply Cyclocary Paliurus, momordica grosvenori and Vc can the vitality of SOD, GSH-Px and increase the competence which can resist the combat of free radical. And it can decrease the amount of MDA to maintain the normal form and function of cells. And the effect of Cyclocary is best than momordica grosvenori and Vc. Conclusion: according to the result of the experiment and prescription of Functional Evaluation Procedure and Testing Method of Healthy Food, under the coordination of exercise and training, taking Cyclocary Paliurus, momordica grosvenori and Vc can obviously decrease the damnification of exercise on mice’s cardiac muscle, liver and improve the function of anti-oxygenation of free ratical and decrease the blight of training on some organics to improve the competence of aerobics. And the effect of Cyclocary Paliurus is best than momordica grosvenori and Vc.更多还原